比较熟地黄不同炮制品引起大鼠滋腻碍胃现象的实验研究

Experimetal study of different processed products of Shudihuang on bored of growing stomach in rats

目的比较熟地黄的4种不同炮制品引起大鼠滋腻碍胃现象的程度。方法选取熟地黄的4种不同炮制品,分别命名为熟地黄-A(清蒸熟地黄)组、熟地黄-B(酒炖熟地黄)组、熟地黄-C(九蒸九晒熟地黄)组、熟地黄-D(九蒸九晒(缺砂仁)熟地黄)组。制备熟地黄-A、B、C、D组水煎液,SD大鼠连续灌胃14 d后,禁食24 h,以碳末半固体糊每只0.5 g、灌胃30 min后,腹主动脉采血,取上清液,用酶联免疫吸附法测定血清胃泌素、血浆胃动素以及P物质的含量。同时,计算胃内容物残留率。结果大鼠胃内容物残留率:空白组为(3.18±0.22)%,熟地黄-A、熟地黄-B、熟地黄-C和熟地黄-D组分别为(3.78±0.27)%,(1.90±0.16)%,(2.40±0.78)%,(2.65±0.67)%。与空白组比较,熟地黄-A组显著增加,而熟地黄-B组、熟地黄-C组、熟地黄-D组显著降低,差异均有统计学意义(均P<0.01)。与熟地黄-A组比较,熟地黄-B组显著降低,差异有统计学意义(P<0.01)。胃泌素含量:空白组为(146.19±123.51)pg·mL^(-1),熟地黄-A、熟地黄-B、熟地黄-C和熟地黄-D组分别为(131.50±59.94),(151.97±120.49),(233.46±15.99),(179.18±36.02)pg·mL^(-1)。与空白组比较,熟地黄-A的胃泌素含量降低,而熟地黄-C组、熟地黄-D组的胃泌素含量显著增加,差异均有统计学意义(均P<0.01)。与熟地黄-C组比较,熟地黄-D组、熟地黄-A组、熟地黄-B组的胃泌素含量显著降低,差异有统计学意义(均P<0.01)。与熟地黄-A组比较,熟地黄-B组的胃泌素含量显著降低,差异有统计学意义(P<0.01)。胃动素含量:空白组为(84.14±125.93)pg·mL^(-1),熟地黄-A、熟地黄-B、熟地黄-C和熟地黄-D组分别为(81.91±313.77),(87.30±67.10),(188.68±32.59),(170.08±83.08)pg·mL^(-1)。与空白组比较,熟地黄-C组、熟地黄-D组含量显著增加,差异有统计学意义(均P<0.01)。与熟地黄-C组比较,熟地黄-D组、熟地黄-A组、熟地黄-B组的胃动素含量显著降低,差异有统计学意义(均P<0.01)。P物质含量:空白组为(19.82±13.87)pg·mL^(-1),熟地黄-A、熟地黄-B、熟地黄-C和熟地黄-D组分别为(13.38±6.47),(23.01±10.41),(37.46±0.44),(29.93±7.77)pg·mL^(-1)。与空白组比较,熟地黄-A组大鼠P物质含量降低,而熟地黄-B组、熟地黄-D组的SP含量增加,差异均有统计学意义(均P<0.05);熟地黄-C组大鼠SP含量也显著增加,差异有统计学意义(P<0.01)。与熟地黄-C组比较,熟地黄-D组、熟地黄-A组、熟地黄-B组大鼠P物质含量显著降低,差异均有统计学意义(均P<0.01)。结论清蒸熟地黄引起大鼠出现滋腻碍胃现象最为严重;而九蒸九晒熟地黄通过提高GAS、MTL、SP的含量以及降低胃内容物残留率,可以有效地消除对大鼠滋腻碍胃的影响。

Objective To observe and compare the effects of 4 kinds of different processed products of Radix Rehmanniae （RR） Preparata on the phenomenon of bored of growing stomach in rats. Methods Four kinds of different processed products of prepared RR were selected and named as following： Shudihuang - A （ steamed RR） ; Shudihuang - B （ stewed with wine RR Preparata and RR） ; Shudihuang - C （ nine steamed nine sun Shudihuang RR Preparata） ; Shudihuang - D [ RR nine steamed nine sun （ Que Sharen） ] . The Shudihuang decoction in A, B, C, D groups were preparation. SD rats were intragastrically administrated for 14 d after fasting 24 h with carbon semisolid paste each 0. 5 g by gavage. After 30 min, the gastrin in serum, plasma motilin and content of substance P were determined by ELISA. After blood collection, the abdominal cavity was exposed, and the gastric net weight was calculated. Results The residual rate of gastric contents in rats of blank group was （3. 18 ± 0. 22） %, the residual rate of gastric contents in rats of Shudihuang - A, - B, - C, - D groups were （3.78 ±0.27）%, （1.90 ±0.16）%, （2. 40 ± 0. 78 ） %, （2. 65 ± 0. 67 ） % respectively with significance（ P 〈 0. 05 in A - group, P 〈 0. 01 in B, - C, - D groups）. Compared with A -group, the difference in B -group with significance（P 〈0. 01 ）. The content of gastrin in blank group was （146. 19 ± 123.51 ） pg · mL-1, the content of gastrin in rats of Shudihuang - A, - B, - C, - D groups were （131.50 ±59. 94）, （151.97 ± 120. 49）, （233.46 ± 15.99）, （179. 18 ±36. 02） pg · mL-1 respectively with significance（P 〈 0. 05 in Shudihuang- A group, P 〈 0. 01 in Shudihuang- C, -D groups）. Compared with C -group, the difference in D-, A-, B -groups with significance（ all P 〈 0. 01 ）. Compared with A -group, the difference in B - group with significance （ P 〈 0. 01 ） . The plasma motilin content in rats of blank group was （84. 14 ± 125.93）pg· mL-1, the plasma motilin content in rats of Shudihuang -A, -B, -C, -D groups were （81.91±313.77）, （87.30 ±67.10）, （188.68 ±32.59）, （170.08 ±83.08）pg· mL-1 respectively with significance（ all P 〉 0.05 Shudihuang - A, - B groups; all P 〈 0. 01 in Shudihuang - C, - D groups）. Compared with C -group, the difference in D -, A -, B -groups with significance（ all P 〈 0. 01 ）. The substance P content of in rats of blank group was （ 19. 82 ± 13.87） pg · mL^-1, the substance P content of in rats of Shudihuang - A, - B, -C, -D groups were （13.38 ±6.47）, （23.01 ± 10.41）, （37.46 ±0.44）, （29.93±7.77） pg· mL^-1 with significance（P 〈 0. 05 in Shudihuang- A group with decreased while Shudihuang- B, -D groups with increased; P 〈0. 01 in Shudihuang -C group with increased）. Compared with C - group, the difference in D - , A - , B - groups with significance （ all P 〈 0.01 ）. Conclusion The bored of growing stomach phenomenon caused by steamed Shudihuang is most serious in rats. The nine steamed nine sun Shudihuang can effectively eliminate greasy effect in rats by increasing the contents of gastrin, motilin, substance P and reducing the residual of gastric con- tents in rate.