细胞间黏附分子-1与小鼠新生血管的相关性研究

Relationship between intercellular cell adhesion molecule-1 and angiogenesis in mice

目的研究细胞间黏附分子-1(ICAM-1)和新生血管在动脉粥样硬化中的表达情况的相关性研究。方法 8周龄Apo E-/-小鼠给予高脂饮食喂养分别至16,24,32周龄,建立代表动脉粥样硬化病变自起始至脂肪纹、纤维斑块及粥样斑块形成不同病变阶段的实验动物模型,取小鼠血清,全自动生化分析仪测定总胆固醇(TC)、低密度脂蛋白胆固醇(LDL)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL),通过免疫组化的方法检测动脉粥样硬化斑块内ICAM-1的表达量。用CD31抗体免疫组化标记新生血管。结果随着周龄增加,TC和LDL逐步增加,8,16,24,32周龄小鼠TC分别为(1.48±0.13),(3.56±0.25),(8.95±0.33),(12.23±0.58)mmol·L^(-1),LDL分别为(0.65±0.06),(2.36±0.18),(4.19±0.31),(5.64±0.55)mmol·L^(-1),与8周龄相比,差异均有统计学意义(P<0.05)。ICAM相对表达量随周龄逐步增加,8,16,24,32周龄小鼠ICAM-1分别为0.05±0.02,1.25±0.35,2.56±0.46,5.23±0.17;8,16,24,32周龄小鼠新生血管数分别为2.12±0.56,7.17±2.64,12.86±3.72,16.78±3.52,与8周龄相比,差异均有统计学意义(P<0.05)。ICAM-1与新生血管呈正相关(y=2.79 x+3.40,r2=0.927 9)。结论动脉粥样进展过程中,ICAM-1与新生血管表达量逐步增加,ICAM-1的表达增加可能是导致动脉粥样新生血管增加的原因。

Objective To study the correlation between the expression of intercellular cell adhesion molecule - 1 （ ICAM - 1 ） and angiogenesis in atherosclerosis. Methods Eight week old ApoE-/- mice were fed with high fat diet up to 16, 24, and 32 weeks respectively, to establish an experimental animal model for the formation of atherosclerotic lesions from the beginning to the fatty streaks, fibrous plaques and atherosclerot- ic plaques. Determination the total cholesterol （TC）, low density lipoprotein （ LDL）, triglyceride （ TG）, high density lipoprotein （HDL） by automatic biochemical analyzer, ICAM - 1 in atherosclerotic plaque was detected by immunohistochemistry. Immunohistochemistry labeling of neovascularization with CD31 antibody. Results TC and LDL increased gradually with the increase of age. The TC in 8,12,24,32 week mice were （1.48 ±0.13）, （3.56 ±0.25）, （8.95 ±0.33）, （12.23 ±0.58） mmol·L^-1. The LDL in 8,12,24,32 week mice were （0. 65 ±0. 06）, （2.36±0.18）, （4.19 ±0.31） significant difference compared with （5.64 ±0.55） mmol ·L^-1, had 8 week old mice （ P 〈 0.05 ）. The relative expression of ICAM - 1 increased gradually with age. The ICAM - 1 of 8, 16, 24, 32 week old mice were 2. 12 ± 0. 56, 7. 17 ± 2.64, 12. 86 ± 3.72, 16. 78 ± 3.52. The number of new vessels of 8, 16, 24, 32 week old mice were 2. 12 ±0. 56, 7. 17 ±2. 64, 12. 86 ±：3.72, 16. 78 ±3.52, had significant difference compared with 8 week old mice （P 〈 0. 05 ）. ICAM - 1 was positively correlated with neovascularization （ y = 2. 79x ± 3.40, r2 = 0. 927 9 ）. Conelttsion In the process of atherosclerosis, the expression of ICAM - 1 and angiogenesis gradually increased, and the increased expression of ICAM - 1 may be the reason of the increase of new blood vessels.