高效帕金森病大鼠模型建立方法的研究

Efficient Methods of Establishing Rat Model of Parkinson's Disease

为了探索高效的帕金森病大鼠模型的建立方法,通过黑质致密部(substantia nigra pars compacta,SNc)、中脑腹侧背盖区(ventral tegmental area,VTA)双位点注射和内侧前脑束(medial forebrain bundle,MFB)单位点注射6-羟基多巴胺2种方法建立帕金森病大鼠模型;术后连续4周运用阿朴吗啡旋转试验鉴定模型是否成功,并用免疫组化方法验证大鼠的SNc多巴胺能神经元的损毁程度。结果表明:SNc-VTA组造模后1周成功率为12%,4周后成功率为72%,MFB组造模后1周成功率即达到84%,2周后的成功率则达到92%;采用2种方法均可成功建立帕金森病大鼠模型,但MFB单位点注射法在短时间内具有更高的成功率。

To explore the best method of establishing a rat model of Parkinson＇ s disease, the rat models of Parkinson＇ s disease were established by the methods of injecting 6 - hydroxydopamine into two regions of brain （ substantia nigra pars compacta（SNc） and ventral tegmental area（VTA） ） and into only one region （medial forebrain bundle, MFB）. Apomor- phine-induced rotation test was performed to verify whether the rat model was succeed in the next 4 weeks, and immunohistochemistry was used to detect the lesion to dopaminergic neurons in the substantia nigra. The results show that the success rate of SNc - VTA group is 12% at the first week and 72% at the 4th week. The success rate of MFB group is 84% at the first week and 92% at the 2nd week. The rat models of Parkinson＇ s disease can be established successfully by both of the methods, but the method with MFB has a higher success rate.