三维打印β-TCP颌骨修复支架的生物学评价

Biological Evaluation of Three-dimensional Printed β-TCP Scaffold for Jaw Reconstruction

目的:探讨三维打印β-磷酸三钙(β-Tricalcium Phosphate,β-TCP)颌骨修复支架的生物学特性及体内成骨作用。方法:采用自动注浆技术制作β-TCP支架,将前成骨细胞(MC35T3-E1)接种在支架上,扫描电镜(SEM)观察材料结构与细胞黏附,CCK-8法检测细胞增殖,ALP法检测碱性磷酸酶活性。将2种支架复合重组人骨形成蛋白-2(recombinant human bone morphogenetic protein-2,rhBMP-2)后植入大鼠体内,发泡法制作的β-TCP支架为对照组,6周后取材行组织学观察。结果:三维打印支架具有规则多孔的立体结构,适合细胞黏附,且增殖及分化能力均高于对照组(P<0.05)。组织学显示复合rhBMP-2后三维打印支架新骨生成量高于发泡法制作的β-TCP支架(P<0.05)。结论:三维打印TCP支架生物相容性良好,复合rhBMP-2后可异位成骨。

Objective：To explore the biological characteristics and in vivo osteogenesis ofβ-tricalcium phosphate（β-TCP）scaffold fabricated by three-dimensional printing（3DP）.Methods：β-TCP scaffolds were fabricated by robocasting.MC3T3-E1cells were seeded onto the scaffold,then scanning electron microscope（SEM）was used to characterize the surface morphology and cells’adhesion.The proliferation and differentiation capability of the cells were assessed by cell counting kit-8（CCK-8）and alkaline phosphatase activities（ALP）.The scaffolds incorporated with recombinant human bone morphogenetic protein-2（rhBMP-2）were implanted into the rats,while foaming-madeβ-TCP scaffolds were used as control.The bone formation was observed by histological examination in 6weeks after operation.Results：The scaffold possessed a well-defined porous stereo-structure.The cell proliferation and differentiation capacity of the 3DP scaffold were significantly higher than those in control group（P〈0.05）.Histological examination showed higher new bone formation of the 3DPβ-TCP than foaming-made scaffold incorporated with rhBMP-2（P〈0.05）.Conclusion：3DPβ-TCP scaffold showed an excellent biocompatibility and ectopic osteogenesis.