摘要
目的:探究载锌多壁碳纳米管/壳聚糖复合材料上的锌离子是否有促进成骨细胞增殖和分化的作用,以及该材料上锌离子的适宜浓度。方法:制备载有不同含量锌离子的多壁碳纳米管/壳聚糖复合材料,并用扫描电镜观察。其中,0.2%(质量百分数)锌含量的复合材料为低锌组,1%(质量百分数)锌含量的复合材料为中锌组,2.5%(质量百分数)锌含量的复合材料为高锌组,不含锌的复合材料为对照组。将MC3T3-E1细胞接种于4种材料表面进行培养,分别用MTT法、碱性磷酸酶试剂盒、ELISA法、激光共聚焦显微镜检测MC3T3-E1细胞在复合材料上的增殖量、碱性磷酸酶活性、骨钙素水平、细胞形态。结果:扫描电镜观察到该材料具有大量孔隙结构;mtt检测中24h、48h、72h时,中锌组细胞增值量高于对照组(P<0.05),低锌组细胞增值量与对照组无明显差距,高锌组细胞增值量小于对照组(P<0.05);碱性磷酸酶活性检测中24h、48h、72h时,中锌组高于对照组(P<0.05),低锌组与对照组无明显差距,高锌组小于对照组(P<0.05);骨钙素水平检测中48h时,中锌组骨钙素浓度高于对照组(P<0.05),低锌组骨钙素浓度与对照组无明显差距,高锌组骨钙素浓度小于对照组(P<0.05);激光共聚焦观察到,在细胞接种48h后,中锌组细胞伸展情况最好,低锌组细胞伸展情况较中锌组差,高锌组细胞伸展较差并且有部分细胞皱缩,对照组细胞伸展情况与低锌组无明显差异。结论:中锌组复合材料更有利于MC3T3-E1细胞的增殖和分化。
Objective:To explore whether zinc ions on the multiwalled carbon nanotubes/chitosan composite can promote proliferation and differentiation of osteoblast,and the appropriate concentration of zinc ion on the materials.Methods:The multiwalled carbon nanotubes/chitosan composites with different contents of zinc ions were prepared,and observed by scanning electron microscope.Thereinto,0.2%zinc content of the composite was low-zinc group,1% zinc content of the composite was medium-zinc group,2.5% zinc content of the composite was high-zinc group,and the composite without zinc was control group.The MC3T3-E1 cells were seeded on surface of the four kinds of composites.Using MTT methods,alkaline phosphatase assay kit,ELISA,and laser scanning confocal microscope,cell proliferation,alkaline phosphatase activity,osteocalcin level,and cell morphology were detected.Results:Scanning electron microscope picture showed that the composite material had a large number of pore structure.The proliferation of cells at 24 h,48hand 72 hin the medium-zinc group was higher than that in the control group(P〈0.05),the low-zinc group was not significantly different from control group,and the high-zinc group was less than control group(P〈0.05).Alkaline phosphatase activities at 24 h,48h,72 hin the medium-zinc group were higher than those in the control group(P〈0.05),the low-zinc group was not significantly different from control group,and the high-zinc group was less than control group(P〈0.05).Osteocalcin level at 48hin the medium-zinc group was higher than that in the control group(P〈0.05),the low-zinc group was not significantly different from control group,and the high-zinc group was less than control group(P〈0.05).Conclusion:The middle-zinc-group composites was more favorable to the proliferation and differentiation of MC3T3-E1cells.
作者
秦博恒
杨犇
何东宁
QIN Bo -heng YANG Ben HE Dong-ning(Shanxi Medical University, Taiyuan 030001, China School and Hospital of Stomatology, Shanxi Medical Univercity, Taiyuan 030000, China.)
出处
《口腔医学研究》
CAS
北大核心
2017年第7期725-728,共4页
Journal of Oral Science Research
基金
山西省卫生与计划委员会资助项目(编号:2015112)
关键词
锌
组织工程骨
增殖
分化
Zinc Tissue engineering bone Proliferation
Differentiation