不同管径二氧化钛纳米管对骨髓间充质干细胞生物学行为的影响

Effects of Titania Nanotubes with Different Diameters on Bone Marrow Mesenchymal Stem Cells

目的:研究不同管径二氧化钛纳米管(TNTs)对骨髓间充质干细胞(BMSCs)生物学行为的影响。方法:阳极氧化法分别制备管径30、100和200nm的TNTs,MTT法检测细胞在材料表面粘附及增殖情况,碱性磷酸酶活性、胶原分泌量和细胞外基质矿化水平检测来评估细胞的成骨分化能力。结果:30nm的TNTs促进BMSCs的早期粘附和增殖,但是其后期细胞增殖和成骨分化能力同对照组相比没有明显的统计学差异;200nm的TNTs明显促进了BMSCs的成骨分化,但是其细胞粘附和增殖明显受到了抑制;100nm的TNTs上BMSCs的早期粘附和增殖受到了轻微的抑制,但是随着时间的推移,其细胞增殖迎头赶上,而且其成骨分化能力显著大于对照组。结论:最适BMSCs生物学活性的应该是100nm的TNTs。

Objective：To investigate the effects of titania nanotubes（TNTs）with different diameters on bone marrow mesenchymal stem cells（BMSCs）.Methods：TNTs with three different diameters were fabricated on Ti surfaces through electrochemical anodization.MTT method was used to determine the cell adhesion and cell proliferation abilities.Alkaline phosphatase activity,collagen secretion,and extracellular matrix mineralization were used to evaluate the osteogenic differentiation of BMSCs.Results：TNTs with a diameter of 30 nm significantly promoted the adhesion and proliferation of BMSCs at the early stage,while there was no obvious difference at the later stage.TNTs with a diameter of 200 nm showed the best ability to promote the osteogenic differentiation.However,it clearly impaired cell adhesion and proliferation.The initial cell adhesion and cell proliferation on TNTs with a diameter of 100 nm were slightly repressed.Furthermore,they could promote the osteogenic differentiation.Conclusion：The optimal diameter of TNTs for BMSCs migth be 100 nm.