Gab2在卵巢癌中的表达及其与铂类药物化疗耐药的相关性研究

Correlation between Gab2 expression in ovarian cancer and resistance to platinum drugs chemotherapy

目的通过检测Gab2在卵巢癌中的表达,探讨其与卵巢癌铂类化疗耐药的相关性。方法利用免疫组织化学及蛋白质印迹法(Western blotting)对该院妇产科2011年1月至2015年6月手术切除的107例患者浆液性卵巢癌组织、12例膀胱癌根治术中切除正常卵巢组织中Gab2的表达进行检测,分析Gab2表达与临床分期,以及与以铂类药物为基础的联合化疗疗效的相关性。结果卵巢癌组织中Gab2表达水平明显高于正常卵巢组织,且与肿瘤的国际妇产科联盟(FIGO)临床分期有关,分期越晚表达越高,差异均有统计学意义(P<0.05);61例卵巢癌患者化疗敏感,46例发生耐药,化疗耐药患者Gab2表达水平明显高于化疗敏感者,差异有统计学意义(P<0.05)。结论 Gab2可能成为预测铂类药物化疗敏感性的有效指标之一。

Objective To investigate the correlation between Gab2 expression and platinum drugs chemotherapy resistance by detecting the expression of Gab2 in ovarian cancer. Methods Immunohistochemical and Western blotting techniques were used to detect the expression of Gab2 in 107 specimens of serous ovarian tumor tissue and 12 specimens of normal ovarian tissues resec- ted during bladder cancer radical operation in the department of gynaeco!ogy of our hospital from January 2011 to June 2015. The correlation between Gab2 expression with clinical stage and effect of combined chemotherapy based on platinum drugs was ana- lyzed. Results The Gab2 expression level in ovarian cancer tissue was significantly higher than that in normal ovarian tissue, more- over had a relation with the FIGO clinical stage,the expression level of Gab2 increased with the increase of clinical stage,the differ- ence was statistically significant （P〈0. 05）; 61 cases were sensitive to chemotherapy, 46 cases were resistant to chemotherapy, the Gab2 expression level in the patients with chemotherapy resistance was significantly higher than that in the patients with chemo- therapy sensitivity,the difference was statistically significant （P〈0.05）. Conclusion Gab2 may become one of effective indexes for predicting platinum drugs chemotherapy sensitivity.