膜联蛋白Ⅱ在肺癌组织、肺泡灌洗液和胸腔积液中的表达及价值

Expression level of Annexin Ⅱ in lung cancer tissue, bronchoalveolar lavage fluid and pleural effusion and its value in the diagnosis of lung cancer

目的 探讨膜联蛋白Ⅱ（AnnexinⅡ）在肺癌组织、肺泡灌洗液（BALF）和胸腔积液中的表达及在肺癌诊断中的价值.方法 选取2014年1月至2016年3月在我院收治的肺癌伴有胸腔积液患者80例（肺癌组）、良性胸腔积液患者40例（良性组）,检测并比较两组患者胸腔积液、BALF中AnnexinⅡ的表达水平;收集肺癌术后组织标本60例、收集正常肺组织标本30例,采用免疫组化染色方法比较两组患者AnnexinⅡ蛋白的表达差异.结果 肺癌组织中AnnexinⅡ的阳性表达率61.67％ （37/60）高于正常肺组织的23.33％ （7/30）,差异具有统计学意义（P＜0.01）;肺癌组织中AnnexinⅡ的阳性表达与淋巴结转移、临床分期有关（P值分别为0.039、0.029）;肺癌组患者的胸腔积液、BALF中AnnexinⅡ表达水平均高于良性组（P均＜0.01）;当胸腔积液中AnnexinⅡ诊断肺癌的临界值为10.94μg/L时,诊断肺癌的灵敏度为83.47％、特异度为80.66％、AUC值为0.837;当BALF中AnnexinⅡ诊断肺癌的临界值为52.08μg/L时,诊断肺癌的灵敏度为86.19％、特异度为89.22％、AUC值为0.898.结论 AnnexinⅡ在肺癌组织、肺癌患者的BALF和胸腔积液中的表达水平均增高,并且对于鉴别诊断肺癌具有一定的临床价值.

Objective To investigate the expression level of Annexin Ⅱ in bronchial lung cancer tissue,alveolar lavage fluid（BALF） and pleural effusion and its value in the diagnosis of lung cancer.Methods From January 2014 to March 2016 in Jingzhou Infectious Disease Hospital,80 cases of patients with pleural effusion of lung cancer （Lung Cancer Group）,40 patients with benign pleural effusion （benign group） were chose.The expression level of Annexin of two groups of patients with pleural effusion,BALF were detected and compared.Sixty specimens of department of pathology past （after lung cancer surgery cancer） and 30 cases of previously collected normal lung tissues （normal lung tissue） were collected,differential expression by immunohistochemical staining were compared between the two groups of Annexin Ⅱ protein.Results The positive expression of Annexin Ⅱ in lung cancer was 61.67% （37/60）,higher than that in normal lung tissue of 23.33% （7/30）,the difference was statistically significant（P〈0.001）.The positive expression of Annexin Ⅱ in lung cancer patients was significantly correlated with rate of lymph node metastasis and clinical stage of lung cancer（P =0.039,0.029）.Group of patients with pleural effusion,BALF expression of Annexin Ⅱ the level was higher than that in benign group（P〈0.001）.When the critical value of Annexin in pleural effusion of lung cancer diagnosis was 10.94 μg/L,the sensitivity of diagnosis of lung cancer was 83.47%,the specificity was 80.66%,AUC value was 0.837.The critical value when BALF Annexin Ⅱ in the diagnosis of lung cancer was 52.08 μg/L,the sensitivity of diagnosis of lung cancer was 86.19%,the specificity was 89.22%,the AUC value was 0.898.Conclusion The expression level of Annexin Ⅱ in the BALF and pleural effusion of patients with bronchial lung cancer and lung cancer is increased,and it has a certain clinical value for the differential diagnosis of lung cancer.