青藤碱诱导的imDC对同种异基因大鼠肾移植外周血Th1／Th2细胞因子的影响

Effect of immature dendritic cells （imDC） induced by sinomenine on the peripheral blood Th1/Th2 cytokines of allogeneic rat renal transplantation

目的观察青藤碱（sN）诱导的供体来源未成熟树突状细胞（imDC）对受体鼠静脉血Th1／Th2细胞因子的影响，探讨SN诱导的imDC诱导移植免疫耐受的机制。方法分别选用Wistar和SD大鼠作为肾移植供体和受体，术前受体经静脉输注imDC，利用显微外科血管吻合法建立大鼠单肾移植模型，以ELISA法测定IL-2、IL-4、IL-10及干扰素（IFN）-γ等的水平。结果（1）移植手术成功率89．5％，动脉吻合时间为（12．5±5．7）min，静脉吻合时间为（17．34-3．4）min。（2）SN—imDC10。组IL-2、IFN-γ、IL-4I及L-10含量分别为（17．25±3．41）pg／ml，（239．80±9．06）pg／ml，（337．60±25．07）pg／ml及（1432．00±106．39）pg／ml。同浓度中，SN—imDC组IL4及IL—10水平高于imDC组及对照组（P〈0．05），且SN—imDC10。组高于SN—imDC10^5组（P〈0．05）；同浓度中，SN—imDC组IL-2、IFN-γ水平低于对照组及imDC组（P〈0．05），且SN—imDC10。组低于SN—imDC10^5组（P〈0．05）。结论（1）利用显微外科血管吻合法可以成功地建立起大鼠单肾移植模型。（2）SN诱导的特异性im—DC可使诱导受体大鼠向Th2型免疫应答的偏移，表明由SN诱导的imDC可能可以介导受体发生免疫耐受。

Objective To investigate the effect of immature dendritic cell （imDC） derived from donor-derived bone marrow induced by alkaloid sinomenine （SN） on the Th1/Th2 eytokines in venous blood of receptors, and to probe into the mechanism which imDC induced by SN can lead transplantation immune tolerance. Methods Inbred strain Wistar and Sprague Dawley （SD） rats were selected as kidney trans-plant donor and recipient, respectively. Vessel sutures of the microsurgery technique were used to build the bilaterally renal transplantation model of rats. By the injection of imDC to the recipient rats preoperatively, enzyme-linked immunosorbent assay （ELISA） was used to determine the level of the interleukin （IL）-2, IL-4, IL-10 and interferon-γ （INF-γ）. Results （1） Successful rate of transplantation was 89. 5%. Arterial anastomosis time was （12. 5±5.7）min,and venous anastomosis time was （17.3±3.4）min. （2） The content of the IL-2, INF-γ, IL-4 and IL-10 in SN-imDC 106 group was （ 17.25±3.41 ） pg/ml, （239. 80±9. 06）pg/ml, （337.60±25.07）pg/ml, and （ 1 432. 00±106. 39）pg/ml, respectively. Among the same concentration, the level of the IL-4 and IL-10 that stood for the Th2 cytokines was significantly higher in SNimDC group than imDC group and control group （P 〈 0. 05 ）, and was significantly higher in SN-irnDC 106 group than SN-imDC 105 group （ P 〈 0. 05 ）. Among the same concentration, the lever of IL-2 and INF-γ that stood for the Thl cytokines was significantly lower in SN-imDC group than imDC group and control group （ P 〈 0. 05 ） , and was significantly lower in SN-imDC 106 group than SN-imDC 105 group （ P 〈 0. 05）. Conclusions （1） The use of microsurgery for anastomosis could make the model of singel kidney transplantation in rats. （2） Specific imDC induced by SN could induce the migration to Th2 immune response, which proved imDC induced by SN could mediate immune tolerance to the recipient.