一株好氧反硝化细菌的分离鉴定及反硝化能力初测

Identification and Denitrification Ability of an Aerobic Denitrifier

采用BTB(溴甲基酚蓝)平板涂布分离法,从鳜鱼养殖池塘水体中分离出17株具有反硝化作用的细菌,通过初步筛选和反硝化能力的测定,挑选出一株具有较强反硝化能力的好氧反硝化细菌,命名为8F-3。菌株8F-3在24 h内将总氮从100.00 mg/L降至6.51 mg/L,去除率达到93.49%;将氨氮从50.000 mg/L降至1.966 mg/L,去除率为96.07%;将硝酸盐氮从50.00 mg/L降至3.51 mg/L,去除率为92.98%;将亚硝酸盐氮从0.096 mg/L降至0.071 mg/L,去除率为25.79%。该试验结果表明,菌株8F-3对氨氮和硝酸盐氮具有较强的去除能力,对亚硝酸盐氮也有一定去除作用,反硝化能力较强。经生理生化测试和16 SrRNA分子鉴定,初步鉴定该菌属于不动杆菌属(Acinetobacter)。

17 denitrification strains were isolated from mandarin fish pond water of Sanshui city using BTB （bromothymol blue） spread-plate technique, through the preliminary screening for denitrification strains and determination ability, one aerobic denitrifying bacteria which has strong determination, was named 8F-3. The results showed that the total content of nitrogen was reduced from 100.00 mg/L to 6.51 mg/L by 8F-3 strain in 24 hours and the rate of denitrification was 93.49%; Ammonia nitrogen was reduced from 50.000 mg/L to 1.966 rag/L, the removal rate was 96.07%, Nitrate nitrogen was reduced from 50.00 mg/L to 3.51 mg/L, the removal rate was 92.98%; Nitrite nitrogen was reduced from 0.096 mg/L to 0.071 mg/L, the removal rate was 25.79%. According to the blast results of 8F-3 strain 16S rRNA sequence （Bootstrap 1 000 times）and physiological test for 8F-3 using API method, the bacteria was finally identified as Acinetobacter sp.