温度和光照对浮游植物叶绿素a提取的影响

Effective of Temperature and Illumination on Extraction of Chlorophyll a in Phytoplankton

采用分光光度法测定浮游植物中叶绿素a的含量,研究光照和温度对提取浮游植物叶绿素a的影响,以确定提取浮游植物叶绿素a的最佳条件。在抽滤过程中严格避光,并用冰水混合物冷却至0℃保存,抽滤水样。浸提分为避光(低于200 lx)冷藏(-1~1℃)组、光照(1 100~1 700l x)冷藏(-1~1℃)组、避光(低于2 0 0 l x)常温(2 6~3 0℃)组和光照(1 100~1 700 lx)常温(26~30℃)组,每组放入10 m L丙酮溶液(90%)浸提19 h。4组平均提取量分别为:避光冷藏组155.73μg/L;光照冷藏组136.24μg/L;避光常温组116.67μg/L;光照常温组89.85μg/L。结果表明浮游植物叶绿素a的最佳提取条件为冷藏、避光。

The spectrophotometric method in different conditions of light and temperature, extraction of phytoplankton chlorophyll a determined, to study the different illumination and temperature on the extraction of phytoplankton chlorophyll a and determine phytoplankton chlorophyll a in the optimum conditions of extraction. Strictly avoid light in the filtration process, and use the ice water mixture is cooled to 0 % preservation, water filtration. Leaching provided to avoid light （less than 200 lx refrigerated（-1-1 ℃ ）group, light（ 1 100-1 700 Ix）cold（-1-1 ℃ ）group, evades the light（less than 200 lx）at room temperature （26-30℃ ） group and light （ 1 100-1 700 lx） at room temperature （26-30 ℃ ） group, each group into the 90% acetone solution extraction 19 h. The average extraction amounts of the four groups were as follows： 155.73 μg/L of the cold storage group and 136.24 μg/L in the light cold storage group, 116.67 μg/L in the ambient temperature group and 89.85 μg/L in the normal temperaW.re group. The results showed that the optimal extraction conditions of phytoplankton chlorophyll a were cold storage and lioht avoidance