中国对虾及中华绒螯蟹多倍体诱导中6-DMAP在卵子中残留量的检测

DETECTION OF 6-DMAP REMAINING IN TREATED EGGS OF PENAEUS CHINENSIS AND ERIOCHEIR SINENSIS IN POLYPLOID INDUCTION

海洋动物多倍体的诱导目前已成为海水养殖中品种改良的重要手段(楼允东,1984)。在美国,太平洋牡蛎和虹鳟的多倍体诱导已大规模应用于生产,并获得良好的经济和社会效益。我国也先后开展了鱼类(桂建芳等,1991;尤锋,1993)、中国对虾(戴继勋等,1993;相建海等,1992)、贝类(喻子牛等,1995)及中华绒螯蟹(陈立侨等,1997)

The remained 6-DMAP in treated eggs during polyploid induction by 6-DMAP in Penaeus chinensis and Eriocheir sinensis was detected using high performance chromatography(HPLC). 100% triploid embryos were induced by inhibiting the second polar body using 300 μmol/L 6-DMAP in fertilized eggs in P. chinensis. Tetraploid of Eriocheir sinensis could be induced using 300 μmol/L 6-DMAP inhibiting the first cleavage, and the highest ratio of tetraploid checked in blastosphere stage was 58% . In HPLC detection, Waters-NOVA PAK C18(5mm× 15mm) was used, and methanol: water (1:1) was used as dilution. 6-DMAP can be detected at 254nm, the minimum of detected 6-DMAP was 0.003μg/ml. The treated eggs rinsed thrice in seawater, the remained 6-DMAP cannot be detected in HPLC at the given condition. During the hatching period, the remained 6-DMAP was diluted and rinsed again and again. 6-DMAP can be degraded in environment. The results indicated that using 6-DMAP to induce polyploid in the eggs of Penaeus chinensis and Eriocheir sinensis is effective and safe.