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凡纳对虾优良性状遗传标记的筛选 被引量:7

IDENTIFICATION OF RAPD MARKERS FOR IMPORTANT TRAITS IN LITOPENAEUS VANNAMEI
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摘要 凡纳对虾(Litopenaeus vannamei)是全球三大养殖对虾名优种类之一。我国从1988年开始试养,到1998年大量引进凡纳对虾亲虾和幼体后,华南地区兴起了养殖热潮。在1999年到2000年短短两年时间内,广东、海南、广西凡纳对虾的养殖面积已达10×10~4亩(1亩=666.7m^2),集约化防病养殖产量普遍达到5~9t/hm^2,年产量高达10~20t/ The genome of Litopenaeus vannamei was analyzed by random amplified polymorphic DNA (RAPD) analysis to identify DNA markers for important traits in Litopenaeus vannamei in the South China. A total of 20 ten-bases primers were screened, and eight positive primers giving highly reproducible RAPD patterns were selected for comparison between healthy and diseased populations of Litopenaeus vannamei. A total of 38 reproducible RAPD fragments ranging from 200 to 1750 base pairs were scored, and 24 fragments (62.2% ) were polymorphic. Only three RAPD markers, OPX - 01 - 1020, OPX - 14 - 530 and OPX - 17 - 940, were found in the diseased population of Litopenaeus vannamei , suggesting the potential use of them as DNA markers for helping broodstock management and selection for breeding.
出处 《海洋科学集刊》 CAS 2002年第1期134-138,共5页 Studia Marina Sinica
基金 中国科学院重大资助项目 B(K295-131-111)号 广东省重点科技资助项目(百项工程) 2KB05503N号。
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