褐牙鲆卵黄囊期能量代谢与生化物质变化特征研究

Biochemical and Metabolic Changes of Embryonic and Larval Development of Bastard Halibut,Paralichths olivaceus

卵黄囊期是鱼类早期发育中的一个重要阶段,是鱼类由内源营养向外源营养转化的一个关键时期。从能量学的角度来分析,则表现为主要能源物质在发育过程中有规律的变化,各能量物质按一定顺序被利用。探讨卵黄囊期海洋硬骨鱼类的能量转化规律,定量分析发育中生化成分和能量物质的变化,预测维持仔鱼生命活动和提供生长所必需的合适饵料营养成分,可为养殖生产中提高早期育苗成活率提供理论依据。作者着重探讨褐牙鲆卵黄囊期能量代谢特点及生化物质变化规律。

Biochemical and morphological parameters of laboratory-reared developing eggs and larvae of bastard halibut (Paralichthys olivaceus) were measured for oxygen uptake ,ammonia excretion, contents of free amino acids(FAA), fatty acids(FA) and accumulated ammonium , and volume of yolk-sac and oil globule at (17.4±0.3)℃.The bastard halibut had an obvious ammonium cumulation during its egg developmental stage. When hatching, the cumulated ammonia were released.Free amino acids (FAA) acted as the most important energy source during the egg developmental stage, and after hatching fatty acids (FA) of neutral lipids in the oil globule was used as the main energy substrate. Before start-feeding, protein began to supply energy base to some extent. Based on our results and accumulated information, it is concluded that the most important energy source has species-specialty in fish developmental biology and its bioenergetics and further research on this theme is urgent for interpretation of early life history pattern during recruitment of marine fishes.