摘要
Left-eyed flounders,Paralichthys olivaceus, were inoculated with inactivated Vibrio anguillarum vaccine via intramuscular injection to analyze level of cellular immune responses induced by the immunogen. The vaccinated as well as control fish were serially sampled for blood cells 2, 3, 4, 5 weeks post immunization to evaluate phagocytosis activity. Changes of phagocytic index (PI) and phagocytic percentage (PP) determined by measurement of ingesting Staphylococcus aureus were observed with Wright’ stain. Results showed that phagocytosis activity began to increase from 2 weeks post immunization. There isare statistically significant differences in PI and PP (P< 0.05) between vaccinated and control fish. The red cell phagocytosis of left-eyed flounders described in the present study for the first time indicates red cell plays an important role not only in respiratory function but also in immune function.
Left-eyed flounders,Paralichthys olivaceus, were inoculated with inactivated Vibrio anguillarum vaccine via intramuscular injection to analyze level of cellular immune responses induced by the immunogen. The vaccinated as well as control fish were serially sampled for blood cells 2, 3, 4, 5 weeks post immunization to evaluate phagocytosis activity. Changes of phagocytic index (PI) and phagocytic percentage (PP) determined by measurement of ingesting Staphylococcus aureus were observed with Wright' stain. Results showed that phagocytosis activity began to increase from 2 weeks post immunization. There isare statistically significant differences in PI and PP (P< 0.05) between vaccinated and control fish. The red cell phagocytosis of left-eyed flounders described in the present study for the first time indicates red cell plays an important role not only in respiratory function but also in immune function.
出处
《海洋科学集刊》
CAS
2007年第1期127-132,共6页
Studia Marina Sinica
基金
国家重点基础研究发展规划项目(973)
G1999012003号
