灵芝孢子油对老龄小鼠免疫保护作用

THE PROTECTING EFFECT OF GANODERMA LUCIDUM SPORES OIL ON IMMUNE FUNCTION IN AGING MICE

目的探讨灵芝孢子油对老龄小鼠的免疫保护作用。方法将192只健康老龄(12月龄)SPF级昆明雄性小鼠随机分为A、B、C、D 4组,各组均随机分为4个小组,即对照组、低、中、高剂量组,每组12只。低、中、高剂量组小鼠经口灌胃分别为0.25、0.50、1.50mg/(kg·bw)的灵芝孢子油,对照组给予同体积的玉米油。灌胃容量0.02 ml/g,1/d。连续灌胃30d后,分别进行迟发型变态反应(DTH)实验、血清溶血素的测定、抗体生成细胞数的测定、碳廓清实验、腹腔巨噬细胞吞噬鸡红细胞实验、淋巴细胞转化实验和NK细胞活性测定。结果灵芝孢子油暴露后,与对照组相比,中、高剂量组小鼠足跖肿胀度、淋巴细胞增殖能力、抗体生成细胞数、抗体积数、单核-巨噬细胞碳廓清能力、腹腔巨噬细胞吞噬鸡红细胞的吞噬率、腹腔巨噬细胞吞噬鸡红细胞的吞噬指数均具有统计学意义(P<0.05或P<0.01),而NK细胞活性无统计学意义(P>0.05)。随着灵芝孢子油灌胃剂量的升高,上述指标均呈上升趋势。结论灵芝孢子油可以增强老龄小鼠的免疫保护作用。

Objective To study the immune protective effect of Ganoderma lucidum spores oil in aging mice. Methods A total of 192 healthy SPF male Kunming aging mice（12 months） were randomly divided into 4 groups. Each group was randomly and equally divided into 4 subgroups, including control group, low, middle and high dose Ganoderma lucidum spores oil groups, given different doses（0.25,0.50,1.50mg/（kg·bw）） of Ganoderma lucidum spores oil respectively at 0.02 ml/g by gavage once a day. The control group was given corn oil with the same volume. At the 30 th day, delayed hypersensitivity test, serum hemolysin. The number of antibody producing cells, carbon clearance test, the phagocytic rate of the peritoneal macrophages to chicken red blood cells, lymphocyte transformation test and NK cell activity assay were carried out. Results After the supplementation of Ganoderma lucidum spores oil, compared with the control group, the feet swollen degree, lymphocyte proliferation ability, antibody producing cell number, antibody titre carbon clearance capacity, phagocytic rate and index of peritoneal macrophages to chicken red blood cell in middle and high dose groups were increased significantly（P〈0.05,P〈0.01）,while NK cell activity was not. With the increase of the dose of Ganoderma lucidum spore oil, the above indexes showed an increasing trend. Conclusion Ganoderma lucidum spores oil can protect immune function in aging mice.