GC-MS法分析红木香药材根和果实中的挥发油成分

Analysis of Volatile Oil from Roots and Fruits of Kadsura longipedunculata by GC-MS

目的:分析红木香药材根和果实中的挥发油成分。方法:采用气相色谱-质谱联用法。色谱条件:色谱柱为HP-5MS石英弹性毛细管柱,载气为高纯氮气,流速为0.9 mL/min,进样口温度为250℃,色谱柱初始温度为50℃(程序升温),柱压为80 k Pa,分流进样,分流比为40∶1,进样量为1.0μL。质谱条件:离子源为电子轰击离子源,电子能量为70 eV,接口温度为230℃,质量扫描范围m/z 40~400,扫描间歇时间为1.0 s。结果:从红木香根的挥发油中总共检出43个色谱峰,鉴定出其中24个,占总量的91.5%;主要成分为倍半萜和单萜类化合物,其中(+)-δ-杜松烯、γ-毕澄茄烯和异朱栾倍半萜相对含量较高。从红木香果实的挥发油中总共检出52个色谱峰,鉴定出其中36个,占总量的82.2%;主要成分为倍半萜和单萜类化合物,其中石竹烯、γ-毕澄茄烯和紫穗槐烯相对含量较高。结论:该研究基本明确了红木香药材根和果实挥发油中的主要成分,并且二者存在明显差异。

OBJECTIVE： To analyze volatile oil from the roots and fruits of Kadsura longipedunculata. METHODS： GC-MS method was adopted. The determination was performed on HP-5MS quartz capillary column with nitrogen as carrier gas at the flow rate of 0.9 mL/min. The temperature of sample inlet was 250 ℃, the initial temperature of column was set at 50 ℃ （temperature programming）. The column pressure was 80 kPa; the ratio of split sampling was 40 ∶ 1. The sample size was 1.0 μL. Mass chromatography was as follows： electron bombardment ion source as ion source; electron energy of 70 eV; inlet temperature was 230 ℃; scanning range of m/z 40-400, scanning interval time of 1.0 s. RESULTS： Totally 43 chromatographic peaks were detected in volatile oil from the roots of K. longipedunculata, and 24 peaks were identified, accounting for 91.5%; main components were sesquiterpenes and monoterpenoids, among which the relative contents of （＋）-delta-cadinene,γ-cadinene and isovalencene were in high level. Totally 52 chromatographic peaks were detected in volatile oil from the fruits of K. longipedunculata, and 36 peaks were identified, accounting for 82.2%; main components were sesquiterpenes and monoterpenoids, among which relative contents of carophyllene, γ-cadinene and valencene were in high level. CONCLUSIONS： The study basically confirm main volatile components in volatile oil from the roots and fruits of K. longipedunculata, and the roots and fruits of K. longipedunculata have significant difference.