原癌基因miR-21与寿命的相关性研究

Relationship between proto-oncogene miR-21 and life span

目的探讨原癌基因miR-21在不同年龄段人群血清中表达量的变化及与寿命的关系。方法选取2014年1月至2016年12月间首都医科大学附属北京友谊医院体检及住院的29例不同年龄段人群为研究对象,其中青年组10例,中老年组9例,长寿组10例,提取3组人员的血清样品,利用实时荧光定量聚合酶链反应(PCR)法检测原癌基因miR-21表达水平,判断miR-21在不同年龄段人群血清中的表达量情况。结果长寿组人员血清中miR-21相对表达量为(0.82±0.39),显著低于中老年组的(1.43±0.51),差异有统计学意义(P<0.05)。将肌酐清除率、EF值和血脂水平分别与miR-21相对含量进行比对发现,不同年龄段miR-21相对含量趋势与血脂水平近似,长寿组人群中血脂水平较中老年组人群略有下降,但差异无统计学意义(P>0.05)。结论原癌基因miR-21可能在长寿人群中表达量减低,抑制恶性肿瘤发生,其表达量与血脂有相关性,有望成为寿命相关性基因之一。

Objective To investigate the expression of proto-oncogene miR-21 in different age groups and its relationship with life span. Methods A total of 29 individuals of different ages were selected at Beijing Friendship Hospital Affiliated to Capital Medical University from January 2014 to December 2016. Among these individuals, 10 were assigned to young group, 9 were assigned to the middle-aged and elderly people group and 10 were assigned to the longevity group. Serum samples were extracted and real-time fluo- rescence quantitative polymerase chain reaction （PCR） method was used to detect the expression level of oncogene miR-21 and determine the the serum expression of miR-21 in different age groups. Results The relative serum expression of miR-21 in the longevity group was （0. 82 ：t： 0. 39 ） which was significantly lower than （ 1.43 ＋ 0. 51 ） of the elderly group （ P 〈 0. 05 ）. After comparing creatinine clearance rate, EF value and serum lipid levels with the relative content of miR-21, it was found that the trend of relative content of miR-21 was similar with the level of serum lipids for different age groups and blood lipid levels were slightly decreased in the longevity group than in the middle-aged and elder group but the difference was not statisti- cally significant （ P 〉 0. 05 ）. Conclusion The expression of proto-oncogene miR-21 may be impaired in the longevity people, which thus may inhibit the malignant tumors, and its expression is related with lipid level, miR-21 is expected to become one of life related genes.