4组沙漠绿藻分子生物学鉴定及其生物学特性研究

Morphological and molecular identification of four groups desert green algae

对从塔克拉玛干沙漠中分离的4组沙漠绿藻进行形态特征鉴定,绘制生长曲线,考察pH对其生长的影响,并进行系统发育分析。形态特征结果显示a组为椭圆形藻,b、c和d组为球状藻。a组沙漠藻最适pH值为6.0,c组的最适pH值以原BG-11培养基为准,b和d组最适pH值为8.0。采用分子生物学18SrDNA序列分析法鉴定出代表性4株沙漠藻的基因序列,结果表明:a组的3YS16(1)和c组3YS21-1的序列跟Chlamydomonas incerta亲缘关系很近;b组的KXII(2)序列跟Chlorosarcinopsis bastropiensis亲缘关系很近;d组的YS2-3的序列跟Chlorosarcinopsis communis亲缘关系很近。该研究确定了4组藻的分类地位和最佳生长pH条件,可为沙漠绿藻分子生物学和食品应用方面的研究提供理论依据。

Four groups of algas, isolated from the Taklimakan desert, were identified by morphological characters in this study. The pH effect of the growth of algae is analyzed according to the growth curve, and the phylogenetic tree was pictured. It shows that the group a belongs to the oval algae, and group b, e and d belong to the spherical algae. The optimum suitable pH values for the group a des err algae was 6.0, 8.0 for the group b and d; the same value of origi hal BG-11 for the group c. Four representative strains of desert algae gene sequences were identified by using the 18S rDNA sequence anal ysis method. It shows that the strain of 3YS16① （group a） and the strain of 3YS21-1 （group c） have close genetic relationship with the Chlamydomonas incerta ; the strain of KXII（2） （group b） has close genetic relationship with the Chlorosarcinopsis bastropiensi; the Strain of YS2 3 （group d） has close genetic relationship with Chlorosarcinopsis comrnunis. This study determined the classification status of four groups of algae and the optimal pH growth conditions, and provided theoretical basis to the molecular biology studies of des ert algae and applications in food industry.