高良姜中4种黄酮化合物的体外抗氧化能力及抑菌活性研究

Study on the antioxidant and antimicrobial activities of four flavonol compounds from Alpinia officinarum Hance rhizomein vitro

从高良姜中提取纯化得到高良姜素、山奈素、山奈酚和槲皮素,以该4种黄酮化合物为原料,对比分析其抗氧化活性,及其对6种食源性腐败菌的抑制作用。结果表明,在抗氧化方面,槲皮素表现出较好的抗氧化活性,山奈酚和山奈素次之,高良姜素最低,但高良姜素在ABTS自由基清除试验中活性与山奈酚和山奈素相似。在抑菌方面,金黄色葡萄球菌对4种黄酮化合物较为敏感,其中枯草芽孢杆菌和金黄色葡萄球菌对槲皮素较为敏感(MIC值分别为1.25,2.50mg/mL,MBC值均为2.5mg/mL);大肠杆菌、白色念珠菌、黑曲霉和绿脓杆菌对4种化合物敏感性低,抑菌活性均不明显。

The in vitro antioxidant and the antimicrobial activities of four flavonol compounds （Galangin, Kaempferide, Kaempferol and Quercetin） extracted and separated of Alpinia officinarum Hance rhizomein were studied. The results showed that querectin had most significant antioxidant activity among the four flavonoids. Moreover,the antioxidant activity of the other three flavonol compounds in order, Kaempferol （highest）, Kaempferide, Galangin （lowest）. How ever the ABTS radical scavenging experiment results showed that the antioxidant activities of the three compounds were the same.The far ther antimierobial experiment resuhs showed thai four flavonol compounds had antimierobial activity to Staphylococcus aurens.S.aureus and Bacillus subtilis were two of the most sensitive among a the lest strains to quercelin, and the MIC and MBC were detected to be 1.25 and 2.5 mg/mL, and 2,5 and 2.5 mg/mL respectively, However all the four compounds showed very weak tesistance against Escherichia coli, P. aeruginosa, Aspergillus mger and Monilia albican.