环氧氯丙烷处理的猪心包瓣组织结构和抗张强度

TISSUE STRUCTURE AND TENSILE STRENGTH OF PORCINE PERICARDIUM MODIFIED WITH EPOXY CHLOROPROPANE

本文对环氧氯丙烷(EC)化学改性前后的猪心包瓣组织进行组织学、超微结构观察和机械抗张强度测试,与牛心包瓣组织比较,发现:(1)猪心包瓣组织是由浆膜层、纤维层和外心包结缔组织层构成;(2)猪心包胶原纤维比牛心包细,但排列致密,同样具有较好的组织抗张强度;(3)组织抗张强度与胶原纤维排列方向有关,胶原纤维以围绕心轴环形纤维方向(环形方向)为主的向异性排列,组织抗张强度以环形方向为最大;(4)用EC化学改性后的猪心包瓣不影响组织结构和抗张强度。这为临床如何裁剪缝制和应用猪心包单瓣补片提供了理论和实验依据。

The incidence of calcification and function ailure of cardiac bioprostheses is rather high after implantation. It is a serious problem affecting the long-term result. Porcine pericardium was modified chemically with epoxy chloropropan (EC) with which monocuspid patches were made and implanted in the outflow tract of right ventricle in dogs. Results showed significanty less calcification with good function of the monocusp. Histology, ultrastructure and mechanical tensile strength of the porcine pericardium were studied before and after modification with EC. Bovine pericardium was used in the control group. It was found thatthe collagenous fibers in porcine pericardium were thinner and their arrangement were denser than those in bovine perilardium; the tensile strength was related to the cdirection of arrangement of the collagenous fibers; porcine pericardium modified with EC had intact tissue structure and tensile strength. Experimental basis for making and using the monocusp of porcine pericardium clinically were presented in this paper.