布渣叶提取物促高脂血症家兔胆固醇逆向转运机制研究

The Mechanism of Microcos Paniculata Extract on RCT in Hyperlipidemia Rabbits

目的:研究布渣叶提取物(MP)的调血脂作用及胆固醇逆向转运(RCT)机理。方法:复制食饵性高脂血症家兔,给予MP(1.4、2.8和5.8g/kg)连续给药6周,检测血清、肝脂质TC、TG、HDL-C和LDL-C含量,肝ApoA1、CYP7A1、LXR-α、LPL及回肠NPCL1L1 mRNA和蛋白表达。结果:MP可显著降低高脂血症家兔血清和肝脏TC、TG(P<0.05),增强肝、回肠ApoA1、CYP7A1、LXR-αand LPL mRNA和蛋白表达(P<0.05),抑制NPC1L1 mRNA和蛋白表达(P<0.05)。结论:MP可通过促进RCT、减少内外源性胆固醇及代谢产物吸收或再吸收,从而调血脂、逆转肝脂肪变性。

Objective： To investigate the lipid regulating effect of Microcos paniculata （MP） extract and the mechanism of reverse cholesterol trans- port （RCT）. Methods： Copied diet-induced hyperlipidemia rabbits and continuously administrated with MP （1.4, 2.8 and 5.8g/kg） for 6 weeks, then detected their contents of serum and hepatic lipid TC, TG, HDL-C and LDL-C, and the mRNA and protein expressions ofApoA1, CYP7A1, LXR-ct, LPL in liver and NPCL1L1 in ileum. Results： MP could significantly reduce the serum and liver TC and TG of hyperlipidemia rabbits （P〈0.05）, en- hance their mRNA and protein expressions of ApoA1, CYP7A1, LXR-ct and LPL （P〈0.05）, inhibit the expressions of NPC1L1 mRNA and protein （P〈0.05）. Conclusion： MP can regulate lipid and reverse liver fatty degeneration via promoting RCT and reducing the absorption or resorption of en- dogenous and exogenous cholesterol and their metabolites.