宫血宁胶囊引起大鼠子宫平滑肌收缩及其初步影响机制研究

Inhibitory effect of Gongxuening on myometrial contractions and mechanisms

目的初步了解宫血宁缩宫胶囊作用的影响机制,为合理应用宫血宁胶囊治疗妇科出血症提供理论指导。方法 Wistar大鼠随机分为宫血宁对照组和宫血宁加相应对比药物组,共9组,每组10只。应用离体子宫张力测定法,测定各组大鼠离体子宫平滑肌收缩活性。结果 0.001μg/ml肾上腺素可完全抑制50μg/ml宫血宁引起的大鼠子宫平滑肌收缩,而肾上腺素β1受体拮抗药阿替洛尔和美托洛尔可完全逆转肾上腺素的抑制作用。肾上腺素α受体拮抗药酚妥拉明虽能抑制α受体激动剂甲氧明引起的大鼠子宫平滑肌的收缩,但不能逆转肾上腺素对宫血宁引起大鼠子宫平滑肌收缩的抑制作用。结论宫血宁胶囊引起的大鼠子宫平滑肌收缩的可能影响机制是通过抑制肾上腺素β受体激活发挥作用。

Objective To investigate the mechanism underlying myometrial contraction stimulated by Gongxuening, and to provide reference for rational use of Gongxuening in the treatment of abnormal uterine bleeding.Methods The contraction of the uterus in vitro induced by Gongxuening in rats was observed using isolated uterine tension measurement, and the influence of such potential factors as epinephrine and its receptor modulators on the contraction was studied.Results It was found that epinephrine at the dose of 0.001μg/ml completely inhibited rat myometrial contractions induced by 50 μg/ml Gongxuening, while β1 adrenergic receptor （β1-AR） antagonists atenolol and metoprolol both completely reversed the inhibition by epinephrine. α-AR antagonist phentolamine could not reverse the inhibition by epinephrine during rat myometrial contractions induced by Gongxuening, although it completely inhibited myometrial contractions induced by α-AR agonist methoamine.Conclusions The possible mechanism of rat myometrial contractions induced by Gongxuening may be through inhibition of epinephrine β- AR activation.