卵巢上皮性癌组织中DNAJB11的表达及临床意义

Expression and clinical significance of DNAJB11 in epithelial ovarian cancer

目的:探讨DNAJB11蛋白在卵巢上皮性癌组织中的表达及其临床价值。方法:采用免疫组织化学EnVision法检测105份卵巢上皮性癌的石蜡包埋组织DNAJB11蛋白的表达情况,并与23份正常卵巢组织、17份卵巢良性肿瘤组织、13份卵巢交界性肿瘤组织对比,用X^2检验分析其与临床病理特征的关系,用Kaplan-Meier法及COX比例风险回归模型分析其与患者生存预后的关系。结果:DNAJB11蛋白在正常卵巢组织及卵巢良性肿瘤中几乎不表达,在交界性肿瘤组织中阳性率为7.7%,在卵巢上皮性癌组织中阳性率为78.1%,卵巢上皮性癌组织中DNAJB11表达均高于其余各卵巢病变和正常卵巢组织(均P<0.01)。在细胞分化程度为G2~G3、临床分期为Ⅲ~Ⅳ期、组织学类型为浆液性、术前外周血CA125水平大于500 IU/L的卵巢上皮性癌患者中,卵巢上皮性癌组织DNAJB11蛋白表达均高于细胞分化程度为G1、临床分期为Ⅰ~Ⅱ期、组织学类型为非浆液性癌和血CA125水平不超过500 IU/L的患者(均P<0.05)。Kaplan-Meier生存曲线显示,DNAJB11强阳性表达与卵巢上皮性癌患者的总生存预后及无瘤生存预后相关(P<0.01);多因素分析结果显示,DNAJB11蛋白强阳性表达是卵巢上皮性癌无瘤生存及总生存的独立预后因素。结论:DNAJB11蛋白可能在卵巢上皮性癌发生发展过程中起重要作用;卵巢上皮性癌组织DNAJB11蛋白强阳性表达是卵巢上皮性癌患者的独立预后因素。

Objective： To ovarian cancer tissues and its study the expression of DNAJBll protein in epithelial clinical significance. Methods： Immunohistochemistry was used to examine DNAJB11 expressions in 105 tissue specimens of ovarian epithelial carcinoma, 23 normal ovarian tissues, 17 tissues of benign tumor, and 13 tissues of borderline tumor. The correlations between protein expression and clinicopathological factors were analyzed by Chi square test. The correlations between protein expression and survival were analyzed by Kaplan-Meier and Cox regression. Results： Positive expression of DNAJB11 protein was observed in O. 0% in normal ovary and benign tumor, 7.69% in borderline tumor, and 78.10% in epithelial ovarian cancer, respectively. Positive expression of DNAJB11 protein was significantly higher in normal ovary and benign tumor than in the rest of the ovarian tissues and normal ovarian tissues （P 〈 0. 001 ）. Higher expression of DNAJBll was more prevalent in tissues from patients with advanced FIGO stages, high serum CA125, poor histological differentiation, and serous cancer. Kaplan-Meier curves revealed that higher expression of DNAJBll was significantly associated with poor disease-free survival and overall survival. Multivariate survival analysis revealed that strong positive expression of DNAJBll was an independent prognostic factor for disease-free survival and overall survival. Conclusion： DNAJBll may play a role in tumorigenesis and progression of epithelial ovarian cancer. Strong positive expression of DNAJB11 was an independent prognostic factor in epithelial ovarian cancer.