利用皮状丝孢酵母对甘蔗渣酶解液产油脂的研究

Oil Production from Enzymatic Hydrolysate of Sugarcane Bagasse by Trichosporon Cutaneum

本试验旨在研究碱和高温液态水2种预处理方式对发酵的影响。甘蔗渣分别利用碱和高温液态水(LHW)预处理,然后使用纤维素酶进行酶解。在LHW和碱预处理后的酶解液中,分别含22.06和39.28 g/L总糖。不经过浓缩,也不需要添加任何营养物质,酶解液被用于皮状丝孢酵母的油脂发酵试验。经过3 d的培养,在LHW预处理的酶解液中,皮状丝孢酵母的生物量达到了8.08 g/L,油脂质量分数为52.00%,油脂系数高达19.03%。在碱预处理的酶解液中,经过8 d的培养,皮状丝孢酵母的生物量为13.67 g/L,油脂质量分数为43.20%,油脂系数为15.03%。脂肪酸组成分析表明所产油脂与棕榈油的组成相似。试验结果表明,甘蔗渣是一种有前景的产油脂原料,LHW是一种良好的预处理方式。该研究为利用甘蔗渣发酵产油脂提供了新思路。

The purpose of this paper aimed to study the effects of two kinds of pretreatment methods （base and liquid hot water） on the fermentation. Sugarcane bagasse was pretreated by base and liquid hot water （LHW）, and then hydrolyzed with cellulase. The enzymatic hydrolysate contained 22.06 g/L and 39.28 g/L sugar respectively pretreated with LHW and base. Without concentration or adding other nutrients, the hydrolysate was used for lipid production using Trichosporon cutaneum. After 3 days, the biomass was 8.08 g/L with lipid content of 52.00% , lip- id coefficient of 19.03% when pretreated by LHW. After 8 days, the biomass was 13.67 g,/L with lipid content of 43.20%, lipid coefficient of 15.03% when pretreated by base. The fatty acid profile of this lipid was similar to that of palm oils. Therefore, sugarcane bagasse was a promising material for oil production, and LHW was a promising pretreated method. This study offered a new thinking for oil production using sugarcane bagasse.