摘要
目的了解福建省II型脊髓灰质炎(脊灰)疫苗相关株病毒(VAPV2)变异特征,为证实阻断VAPV2传播提供依据。方法采用RT-PCR扩增VAPV的VP1全长和部分3D区基因序列,统计分析病毒核苷酸(nt)和氨基酸(aa)变异情况。结果 2000-2015年福建省从5 497份粪便标本中分离到219株脊灰病毒,1株为疫苗衍生脊灰病毒(VDPV),218株为VAPV,其中VAPV2占37.16%。与Sabin2株相比,47株VAPV2中VP1变异位点>5个2株(4.26%),≤2个36株(76.60%);共涉及45个位点,其中nt2909为突变热点,导致VP1的aa143位点从非极性疏水性转换成极性亲水性。13株VAPV在3D区出现重组。结论福建省大部分VAPV2变异少,VP1的aa143位点为突变热点。
Objective To analyze genetic characteristics of type II vaccine-associated polioviruses （VAPV2）, and to provide evidence for certifying interruption of VAPV2 transmission in the future. Methods Gene sequences of the entire VP1 and partial 3D regions of VAPVs were amplified by RT- PCR to identify VAPV2 nucleotide （nt） and amino acid （aa） mutations. Results 219 strains of polio- virus were isolated from 5 497 stool samples in Fujian from 2000 to 2015 ; 1 strain was a vaccine derived poliovirus （VDPV） , and 218 strains were VAPV, among which VAPV2 accounted for 37.16%. Among 47 VAPV2 strains, 2 （4.26%） had 〉5 mutation sites and 36 （76.60%） had ~〈2 mutation sites in the VP1 region compared with Sabin2 strain. A total of 45 mutation sites were involved, in which nt 2909 was a mutation hot spot, resulting in the aa 143 site of VP1 changing from being non-polar and hydropho- bic to being polar and hydrophilic. Thirteen strains were recombinant in 3D region. Conclusion Most VAPV2 isolates in Fujian had few mutations; the aa 143 of VP1 was a mutation hot spot.
出处
《中国疫苗和免疫》
北大核心
2017年第3期262-266,共5页
Chinese Journal of Vaccines and Immunization
基金
福建省卫生系统中青年骨干人才培养项目(2013-ZQN-ZD-10)
