复方苦参汤抗溃疡性结肠炎活性成分的初步研究

Active components of Fufang Kushen decoction for ulcerative colitis

目的研究复方苦参汤抗溃疡性结肠炎的活性成分。方法采用系统溶剂法、硅胶柱层析、中压制备液相、制备液相、重结晶等仪器方法,对复方苦参汤水煎液进行分离纯化和结构鉴定。并通过考察各化合物对LPS刺激RAW 264.7细胞产生的NO的抑制作用,对各化合物的抗NO活性进行筛选。结果从复方苦参汤中分离得到7个化合物,化合物1~7经鉴定分别为苦参碱、氧化苦参碱、靛蓝、靛玉红、没食子酸、甘草苷和甘草酸。并筛选出4个活性成分。结论初步确定复方苦参汤抗溃疡性结肠炎的活性成分为苦参碱、氧化苦参碱、靛蓝及靛玉红。

Objective To determine the active components of Fufang Kushen decoction for ulcerative colitis. Methods The compound decoction of Fufang Kushen decoction was separated and purified by solvent system, silica gel column chromatography, medium pressure liquid phase, semipreparative liquid phase and recrystalliza- tion. The anti-NO activity of the compounds was screened by examining the inhibitory effect of each compound on the NO production stimulated by RAW 264.7 cells. Results Seven compounds were isolated and identified as matrine, oxymatrine, indigo, indirubin, gallic acid, glycyrrhizin and glycyrrhizic acid. For active components were screened out. Conclusion Preliminary determination of the active ingredients of Fufang Kushen decoction against ulcerative colitis includes matrine, oxymatrine, indigo and indirubin.