摘要
目的建立同时检测参须中人参皂苷Rg1、Re和Rb1含量的HPLC-MS/MS方法,测定10批参须中人参皂苷Rg1、Re和Rb1的含量。方法采用液质联用(HPLC-MS/MS)法,色谱柱为SHIMADZU VP-ODS(4.6 mm×150 mm,5μm),流动相为乙腈-0.1%甲酸水,梯度洗脱(0~3 min,60%乙腈;3~5 min,90%乙腈),流速0.5 m L·min-1,柱温40℃。质谱条件:正离子检测模式,用于定量分析的离子对分别为:人参皂苷Rg1 m/z 823.3→643.6,人参皂苷Re m/z 969.7→789.6和人参皂苷Rb1 m/z1131.5→365.3。结果人参皂苷Rg1在0.43~27.6μg·m L^(-1)与峰面积线性关系良好,r=0.9950,平均回收率为94.0%(RSD=1.5%);人参皂苷Re在0.46~29.6μg·m L^(-1)与峰面积线性关系良好,r=0.9990,平均回收率为95.3%(RSD=1.1%);人参皂苷Rb1在0.41~26.4μg·m L^(-1)与峰面积线性关系良好,r=0.9980,平均回收率为93.4%(RSD=1.2%)。结论该方法简便、准确、快速,可用于参须中人参皂苷Rg1、Re和Rb1的含量检测。
Objective To determine ginsenosides Rgl, Re and Rbl in 10 batches of Panax ginseng tails by HPLC-MS/MS. Methods SHIMADZU VP-ODS C18 （4.6 mm- 150 mm, 5 μm） column was used with the mobile phase consisting of acetonitrile-water （0.1% FA）, and the gradient elution （0 -- 3 min, 60% acetonitrile; 3 -- 5 min, 90% acetonitrile） was.used. The flow rate was 0.5 mL min-1 and the column temperature was set at 40 ℃ . MS analysis was monitored in positive mode. Ion pair of substance inquantitative determination was as follows： ginsenoside Rg1 m/z 823.3 →643.6; ginsenoside Re m/z 969.7 → 789.6; ginsenoside Rbj m/z 1131.5 → 365.3. Results The linear range of ginsenoside Rg1 was 0.43 - 27.6 μg mL-1 （r = 0.9950） with recovery of 94.0% （RSD = 1.5%）; that of ginsenoside Re was 0.46 - 29.6 μg mL-1 （r= 0.9990） with recov- ery of 95.3% （RSD = 1.1%）; that of ginsenoside Rb1 was 0.41 - 26.4 μg mL-l （r = 0.9980） with recovery of 93.4% （RSD = 1.2%）. Conclusion This method is simple, accurate and rapid, which can be used for the determination of ginsenosides Rg1, Re and Rb1 in Panax ginseng tails.
出处
《中南药学》
CAS
2017年第6期807-810,共4页
Central South Pharmacy
