昆虫杆状病毒egt基因研究进展

The Research Progress of Baculovirus egt Gene

杆状病毒是一类大分子的双链环状DNA病毒,其编码的蜕皮甾体尿苷二磷酸葡萄糖基转移酶基因(egt)在感染昆虫后表达产生EGT酶。EGT酶的催化使宿主体内的蜕皮激素失活,阻止幼虫蜕皮,从而利于病毒自身的繁殖。已有研究发现,敲除egt基因的杆状病毒感染的昆虫,死亡时间缩短,向上攀爬行为减弱。最近的研究还表明转egt基因的家蚕蚕蛹发育迟缓。从分子水平对EGT酶作用机制及其与宿主蛋白互作关系进行探索,将加深我们对杆状病毒调控宿主生长发育和行为变化策略的认识,有利于农业生产应用(特别是蚕丝业,如鲜茧缫丝),且为新型高效生物杀虫剂的研发提供新思路。

Baculoviruses are characterized by a large circular double-stranded DNA genome, the ecdysteroid UDPglucosyltransferase（EGT） was produced by egt gene after infecting insects. Catalytic activity of EGT can inactivate ecdysteroids, prevent the metamorphosis of larvae, thus contributing to the virus reproduction. Several lines of evidence have shown that the deletion of the egt from the nucleopolyhedrovirus genome shorten the dearth time and eliminate the upward climbing behavior of lepidopteran larval host. A recent study reported that egt expression in egt-transgenic silkworms results in arrest of metamorphosis from pupae to moths. Studies on the molecular mechanism of EGT and its interaction with host proteins will deepen our understanding of the effect of baculovirus on host growth and behavior changes. It is not only conducive to the production of agriculture（especially the silk industry, such as fresh cocoon reeling）, but also provides new ideas for the research and development of new efficient biopesticides.