禽流感疫苗抗原浓缩工艺的研究

Study on the concentration skills of antigen of avian influenza vaccines

为了提升禽流感疫苗中的抗原效价,本研究采用密理博公司的切向流超滤系统,选用不同孔径的超滤膜和浓缩倍数对禽流感病毒(H9N2亚型,HP株)进行浓缩,观察HA和EID_(50)的变化。结果显示:膜包孔径越大,病毒透过越多,但膜包孔径过小,浓缩时间长;随着浓缩倍数增加,HA和EID_(50)均呈正相关增长,但高浓缩倍数耗时较长。说明禽流感疫苗生产中应根据抗原相对分子质量、HA、EID_(50)和生产效率选择合适的超滤膜孔径和浓缩倍数。

In order to increase antigen levels of avian influenza vaccines, avian influenza viruses（H9N2 subtype,HP strain） were con- centrated with difference pore diameter uLtrafihration membrane and difference concentration times by tangential flow filtration systems of Merck Millipore. And the HA and EID50 of avian influenza viruses concentrated were examined to compare and analyze. The resuLts showed that more avian influenza viruses were wasting with increasing of pore diameter of uLtrafihration membrane, but it took more time with decreasing of pore diameter. Also, HA and EID50 increased depending on concentration times, and it took more time with increasing of concentration times. Overall, it shouLd synthetically consider relative molecuLar mass of antigen, HA, EID50 and production efficiency to choose appropriate pore diameter uLtrafiltration membrane and concentration times in production of avian influenza vaccines.