印楝素对min6细胞增殖及胰岛素分泌的影响

Effects of azadirachtin on proliferation and insulin secretion of min6 cells

【目的】研究印楝素对min6细胞增殖、葡萄糖摄取及胰岛素分泌的影响,并探讨其作用机制。【方法】不同浓度印楝素处理min6细胞48 h,检测细胞增殖量。在5.5和25.0 mmol·L^(-1)葡萄糖条件下,检测印楝素处理细胞的葡萄糖摄取水平,胰岛素ELISA检测试剂盒检测细胞胰岛素的分泌。【结果】与对照组相比,在5.5和25.0mmol·L^(-1)葡萄糖条件下,印楝素均表现促进细胞增殖活性。印楝素处理下,与25.0 mmol·L^(-1)葡萄糖条件相比,5.5 mmol·L^(-1)葡萄糖表现出更显著地促进葡萄糖摄取和胰岛素分泌的作用。【结论】印楝素可能通过调控葡萄糖水平影响胰岛素的分泌。

【Objective】 To investigate the effects of azadirachtin on cell proliferation,glucose uptake and insulin secretion in min6 cells and their possible mechanisms. 【Method】 The min6 cells were cultured in vitro and treated with various concentrations of azadirachtin for 48 h.Cell proliferation was measured. In the presence of 5.5 and 25.0 mmol·L^-1 glucose,glucose uptake was measured and insulin secretion in min6 cells were detected by the insulin ELISA kit. 【Result】 Azadirachtin promoted the proliferation of min6 cells in the presence of 5.5 and 25.0 mmol·L^-1 glucose compared to the control group. Compared with the 25.0 mmol·L^-1 glucose condition,azadirachtin in the presence of 5.5 mmol·L^-1 glucose more significantly promoted glucose uptake and insulin secretion in min6 cells. 【Conclusion】Azadirachtin could affect insulin secretion by regulating glucose concentration.