CD44通过重编程SP侧群细胞影响鼻咽癌的进展

CD44 can affect the progression of nasopharyngeal carcinoma by reprogramming the SP side population cells

目的探讨CD44通过重编程侧群细胞和影响鼻咽癌进展的分子机制。方法利用超高速细胞分选平台,鉴定、分选鼻咽癌细胞系SP侧群细胞。从蛋白质水平研究鼻咽癌细胞、SP侧群细胞生物学特性及其影响鼻咽癌进展的分子机制。结果 CD44分子作用网络中CXCR4、CD31、MMP-9在SP细胞中表达上调(P<0.05);CD44及其下游调控分子ZAP70蛋白在SP细胞中表达增高(P<0.05)。MTT细胞加入SP细胞中肿瘤微球的数目显著增加(P<0.05)。CD44分子作用网络中CXCR4、CD31、MMP-9、ZAP70蛋白在SP细胞中表达与肿瘤微球的数目呈正比(P<0.05)。结论 CD44通过重编程SP侧群细胞影响鼻咽癌的进展。

Objective To explore the molecular mechanism of CD44 can affect the progression of nasopharyngeal carcinoma by reprogramming the SP side population cells.Methods Identification and sorting of side population cells of nasopharyngeal carcinoma cell line SP by super high speed cell sorting platform.The biological characteristics of nasopharyngeal carcinoma cell and SP side population and the molecular mechanism of its influence on the progression of nasopharyngeal carcinoma were explored from the protein level.Results CD44,CD31 and MMP-9 were up-regulated in CXCR4 cells （P〈0.05） in SP cells.The expression of ZAP70 protein in SP cells was increased in CD44 and its downstream regulatory molecules（P〈0.05）.The number of MTT cells added to SP cells was significantly increased（P〈0.05）.The expression of CXCR4,CD31,MMP-9 and ZAP70 protein in SP cells were proportional to the number of tumor microspheres（P〈0.05） in CD44 cells.Conclusion CD44 affects the progression of nasopharyngeal carcinoma by reprogramming the SP side population cells.