异属异养硝化菌硝化特征与人工强化互补性研究

Study on the Nitrification Characterization and Artificial Enhancement Complementarity of Heterotrophic Nitrifying Bacteria from Different Genus

选取菌株Flavobacterium sp.FL211T、Acinetobacter sp.AC211B、Massilia sp.MA211S作为出发菌株,探讨p H、碳源、C/N对不同菌属异养硝化菌硝化效率的影响。结果表明,菌株FL211T、AC211B、MA211S的最佳pH分别为9、7、8;菌株FL211T的最佳碳源为葡萄糖,而菌株AC211B、MA211S均无法利用葡萄糖,最佳碳源均为琥珀酸钠;3株菌的最佳C/N分别为20、15、20,在最适条件均具良好的异养硝化性能。3个菌属细菌的最适异养硝化条件有所差异,但可以优势互补,混合投加或制成复合菌剂进行微生物人工强化较纯菌富集投加更具优势。

Strains Flavobacterium sp. FL211 T,Acinetobacter sp. AC211 B and Massilia sp. MA211 S were selected to explore the effects of initial p H,carbon source and C/N on the nitrification of bacteria which belong to different genus. The results showed that the optimum p H of strains FL211 T,AC211B and MA211 S were 9,7 and 8,separately;glucose was the most suitable carbon source for strain FL211 T,and sodium succinate for strains AC211 B and MA211S;the optimal C/N of them was 20,15,20. They all exhibited excellent nitrification ability under the optimum conditions. Although the optimum heterotrophic nitrification conditions of three bacteria vary,the advantages can be complementary,and dosing with mixed bacteria or compound microbial agent has more advantages than adding pure cultured enrichment bacteria in microbial artificial enhancement process.