靶向沉默CD105和Ki67的表达对卵巢癌OVCAR3细胞生物学行为的影响

Impacts of silencing CD105 and Ki67 gene expression on the biological behavior of ovarian cancer OVCAR3 cells

目的探讨沉默CD105和Ki67基因表达对人卵巢上皮癌OVCAR3细胞系生物学行为的影响。方法CD105-siRNA、Ki67-siRNA、CD105-siRNA+Ki67-siRNA、阴性对照组分别转染卵巢癌OVCAR3细胞,用MTT法、划痕实验、Transwell小室及流式细胞术检测CD105、Ki67单基因沉默及联合基因沉默对人卵巢癌细胞增殖、迁移、侵袭及凋亡的影响。结果与各自的空白对照组及空脂质体对照组相比,单基因CD105-siRNA组、单基因Ki67-siRNA组和双基因联合干预组基因沉默后人卵巢癌OVCAR3细胞的增殖能力、迁移能力及侵袭能力均明显下调,其中联合干预组下降最为明显,癌细胞凋亡率明显增加,其中联合干预组增加最为明显,差异存在统计学意义(P<0.01,P<0.05)。结论 CD105-siRNA和Ki67-siRNA表达载体均可抑制人卵巢癌OVCAR3细胞的增殖,并降低其细胞迁移和侵袭能力,诱导其肿瘤细胞凋亡。双基因联合沉默,效果更加显著。

Objective To investigate the effect of CD105 and Ki67 silence on ovarian cancer cell line OVCAR3 cells. Methods OVCAR3 cells were transfected with siRNA of CD105-siRNA ggroup,Ki67-siRNA group,CD105-siRNA＋ Ki67-siRNA group,negative control groups in vitro respectively. Cell proliferation,migration,invasion and apoptosis after transfection were analysed with MTT assay,wound-healing assay,Transwell assay and flow-cytometric. Results Compared with the negative control group,the expressions of mRNA and protein in CD105-siRNA group and Ki67-siRNA group were decreased significantly; Cell proliferation, migration, invasion ability were decreased significantly after transfection; apoptosis was increased. The most significant change was detected in CD105-siRNA ＋ Ki67-siRNA group（ P 〈0. 01,P 〈 0. 05）. Conclusion CD105-siRNA and ki67-siRNA expression vector can inhibit the proliferation of human ovarian cancer OVCAR3 cells and reduce their cell migration and invasion,and induce apoptosis in tumor cells. The effect of the double gene silence is more obvious.