贺兰山紫蘑的分离、鉴定与培养条件

Seperation,Identification and Culture Conditions of Cortinarius sp.

从采集自内蒙古贺兰山青海云杉林下的紫蘑子实体中分离获得纯培养菌丝体,结合形态学特征与ITS序列分析鉴定其分类学地位,研究了菌丝生长的最适培养基、温度和pH,并结合其菌根菌特性,进一步考察了宿主植物青海云杉根、枝、针叶浸提物对菌丝生长的影响。结果表明:Cortinarius sp.菌株的ITS序列与GenBank中报道的采集于加拿大的Cortinarius rufoolivaceus(GenBank登录号为FJ039645)的ITS序列同源性最高,为98%,结合其形态学特征,确定该菌株为丝膜菌属(Cortinarius sp.)真菌;菌丝体生长的最适温度为15~20℃,最适pH为5.5~6.0;合适浓度的青海云杉枝及针叶浸提物对菌丝生长有显著的促进作用,菌丝干质量最大分别达到(8.62±0.27)mg和(6.93±0.27)mg,分别比对照提高了45.85%和17.26%。

A strain of Cortinarius sp.was isolated from a wild mushroom fruiting body under Picea crassifoliaforest,from Helan Mountain,and was identified based on its morphology and ITS sequence analysis.The optimum culture conditions,i.e.medium,temperature,pH and the effects of extraction from the coniferous,branches and roots of Picea crassifolia on the mycelium growth were investigated.The results showed that the strain manifested relatively high sequence similarity with Cortinarius rufoolivaceus（Canada）samples based on polymeric analysis of ITS sequences,with similarity of 98%.Combined with its morphological characteristics,it was therefore identified as Cortinariussp.LM-1.Culture conditions showed that the optimum temperature was 15-20 ℃,and the optimum pH was 5.5-6.0.The branch and needle extraction of the host plant Picea crassifolia had significant roles in promoting the mycelium growth,enhanced the mycelial dry weight（（8.62±0.27）mg and（6.93±0.27）mg）with the value of 45.85% and 17.26% comparing with the control group.