不同产地巴戟天药材中大黄素甲醚的含量测定

HPLC analysis of physcion in Morinda officinalis from different locations

目的:对不同产地巴戟天药材中大黄素甲醚的含量进行比较研究。方法:采用HPLC测定不同产地巴戟天药材中大黄素甲醚的含量,色谱柱:Agilent(4.6 mm×250 mm,5μm);以甲醇-0.1%磷酸水为流动相(78:22);流速1.00 m L·min^(-1);柱温30℃;检测波长254nm。结果:巴戟天药材中大黄素甲醚的线性范围为0.118-1.18μg/m L,r^2=0.9991,大黄素甲醚的平均回收率在87.12%。不同产地巴戟天药材中大黄素甲醚的含量有所不同。结论:所建立的分析方法可以测定巴戟天药材中大黄素甲醚的含量。

Objective ：To establish a HPLC method to determine the quantitative of physcion in Morinda officinalis from different locations. Methods：Separation column was Agilent C18 （4.6×250mm, 5 Ixm）with the column temperature of room temperature. The mobile phase composition was methonal -0. 1% phosphoric acid （78：22） and the flow rate was 1.0ml/min. The UV detection wavelength was 254nm. Results ：A good linearity was obtained in the range of 0. 118 - 1. 18μg/mL with r^2 =0. 9991. The average recovery was87.12%. Conclusion ： The content of physcion was vary with the producing area of Morinda officinalis. The method was good for determining physcion in Morinda officinalis.