免疫亲和柱净化-HPLC法测定植物油中黄曲霉毒素

Detecting of Aflatoxin B_1,B_2,G_1,G_2 in Vegetable Oil by HPLC with Immunoaffinity Columns

目的:建立一种免疫亲和柱净化-高效液相色谱同时测定植物油中黄曲霉毒素B_1,B_2,G_1,G_2的方法。方法:试样用甲醇水(55+45,体积比)提取,提取液经离心、过滤、稀释后,用免疫亲和柱净化,采用高效液相色谱-大体积流通池荧光检测器检测。结果:在优化条件下,黄曲霉毒素B_1,G_1的检出限分别为0.067μg/kg,黄曲霉毒素B_2,G_2的检出限分别为0.02μg/kg,回收率范围为94.0%~105.7%。结论:该方法简便快速,灵敏度高,重复性好,可满足植物油中黄曲霉毒素含量检测的需要。

Objective A method for the detecting of aflatoxin B1 ,B2 ,G1 ,G2 in vegetable oil by HPLC with immunoaffinity columns was established. Methods Samples were extracted with methanol - ater（ 55 ＋ 45 ） solution. After oscillated and centrifugated, he lower layer of extraction was filtered, fluted with water and filtered. The filtrate was then passed through immunoattinity column, hen determined by fluorescence detector with large volume flow cell. Results Under optimum conditions, he limits of detection of aflatoxin Bl ,G1 were 0.067μg / kg,he limits of detection of aflatoxin 82, Glwere 0.02μg / kg,Respectively and the recoveries of aflatoxin were 94.0% ~ 105.7%. The method is simple, apid and accurate, and is applicable for the determination of aflatoxins in vegetable oil.