五倍子抗口腔致病菌活性物质筛选及其体外抑菌效果评价

Screening and evaluation of the active substance of gallnut against oral cavity pathogens

目的:研究3种五倍子提取物对6种常见口腔致病菌生长、代谢的影响。方法:通过一系列提取分离纯化,从五倍子中筛选出3种活性单体,即没食子酸、没食子酸甲酯、没食子乙酯。采用液体稀释法研究这3种五倍子活性单体对变形链球菌、粘性放线菌、牙龈卟啉单胞菌、粪肠球菌、具核梭杆菌及白色念珠菌生长、代谢的影响。结果:没食子酸对6种细菌的MIC值(mg/ml)为2.5~5、没食子酸甲酯为2.0~4.0,没食子酸乙酯为1.25~2.5,浓度为1 mg/ml时,3种提取物均能抑制这6种口腔致病菌生长、产酸及产胞外多糖。且没食子酸乙酯作用最强。结论:没食子酸、没食子酸甲酯、没食子酸乙酯在较低浓度下对口腔主要致病菌的生长、酸代谢和糖代谢都有一定的抑制作用。

Objective： To study the effects of 3 extracts from Chinese gall on the growth and metabolism of 6 kinds of oral bacteria. Methods： Through a series of extraction and purification, 3 active monomer, gallnut extract gallic acid, methyl gallate and gallic acid ethyl ester were selected. Streptococcus mutansUA159 （ UA159 ）, Actinomyces viscosus （ Av ）, Porphyromonas gingivalis （Pg）, Enterococcus faecalis （ Ef） , Fusobacterium nucleatum（ Fn ） and Candida albicans （Ca） were treated by the extracts respectively, The growth and metabolism of the becteria were studied by liguid dilution method. Results： The MIC（mg/ml） of Gallic acid anainst the bacteria was 2.5 - 5, methyl gallate 2.0 - 4.0 and gallic acid ethyl ester 1.25 - 2.5. The extracts at 1 mg/ml could inhibit the growth, acid production and extracellular polysaccharides of the 6 oral pathogens. And ethyl gallate showed the strongest effects. Conclusion： Gallic acid and methyl gallate and ethyl gallate at low concentration may inhibit the growth, acid metabolism and glucose metabolism of oral bacteria.