糖尿病型牙周炎牙龈组织中炎症因子表达及与细胞凋亡关系

The relationship between inflammatory factor expression and cell apoptosis in gingival tissue of the subjects with diabetes associated periodontitis

目的:探讨糖尿病型牙周炎(diabetes associated periodontitis,DAP)牙龈组织中凋亡细胞发生及与IL-1β和TNFα表达的关系。方法:纳入DAP患者和健康龈(H)受试者各20例,用HE染色和Tunnel法观察牙龈细胞凋亡,透射电镜观察凋亡细胞超微结构;用免疫组化(IHC)检测牙龈组织炎症因子IL-1β和TNFα表达。结果:DAP组牙龈上皮棘细胞层和基底细胞层见明显细胞凋亡,固有层凋亡细胞较少;DAP组牙龈上皮棘细胞和基底细胞凋亡百分率高于H组(P<0.01);IHC染色发现DAP组IL-1β和TNFα表达明显高于H组,主要阳性表达细胞为巨噬细胞、浆细胞和淋巴细胞。结论:DAP患者牙龈组织中IL-1β和TNFα在细胞凋亡中起重要作用。

Objective： To study the relationship between IL-1β and TNFα expression and cell apoptosis in gingival tissue of the subjects with diabetes associated periodontitis （DAP）. Methods： 20 cases of DAP（ group DAP） and 20 cases of health controls （ group H） were included. The cell apoptosis and the uhrastructural changes in gingival tissue were observed by Tunnel staining and transmission electron microscope （TEM）. IL-1β and TNFα expression in gingival tissue were detected by immunohistochemical staining. SBI, GI, PD and AL of the subjects were measured. The relationship between the level of IL-1β ,TNFα and the cell appotosis was analyzed. Results： Apoptosis was obvious in prickle cells and basal ceils of gingival tissue of DAP group. The percentage of apoptosis cells of DAP group was significantly higher than that of group H（ P 〈 0.01 ）. The expression of IL-1β and TNFα in group DAP was significant higher than that of group H （P 〈0.01 ）, and the mainly positive expression cells were maerophages, plasmoeytes and lymphocytes. Conclusion： IL- 1β and TNFα play a role in cell apoptosis in the gingival tissue of the patients with DAP.