摘要
[Objective] The paper aimed to get a efficient, stable and low-priced DNA extraction method of mulberry leaves. [Method] With tender leaves of mulberry varieties in Anhui Province as materials, genomic DNA was extracted with CTAB method, and further identified by ultraviolet spectrophotometer, agarose gel electrophoresis and PCR methods. [ Result] The A260/A280 ratio of extracted DNA was 1.80 - 1.90, and the yield was 0. 187 -0.275μg/mg. With extracted DATA as the template, clear target bands were obtained by PCR. [Conclusion] Extracted DNA was good in quality, which could satisfy the needs of follow-up mo-lecular biological operation.
[Objective] The paper aimed to get a efficient, stable and low-priced DNA extraction method of mulberry leaves. [Method] With tender leaves of mulberry varieties in Anhui Province as materials, genomic DNA was extracted with CTAB method, and further identified by ultraviolet spectrophotometer, agarose gel electrophoresis and PCR methods. [ Result] The A260/A280 ratio of extracted DNA was 1.80 - 1.90, and the yield was 0. 187 -0.275μg/mg. With extracted DATA as the template, clear target bands were obtained by PCR. [Conclusion] Extracted DNA was good in quality, which could satisfy the needs of follow-up mo-lecular biological operation.
基金
Supported by Dean Youth Innovation Fund of Anhui Academy of Agricultural Sciences"ITS Sequence and Evolutionary Analysis of Local Mulberry Germplasm Resources in Anhui Province"(15B0634)
Hefei Comprehensive Test Station of National Sericulture Technology System"Special Project for Construction of China Agricultural Industry Research System"(CARS-22-SYZ09)
Special Talent Development Fund of Anhui Academy of Agricultural Sciences"Breeding of High Quality Ecological Mulberry Varieties and Integration and Demonstration of Matching Cultivation Technology"(17F0610)
Discipline Construction of Anhui Academy of Agricultural Sciences"Research of Silkworm Gender Tag Technology"(16A0618)
