摘要
目的:分析39例强直性脊柱炎患者和20例健康体检者的血清免疫球蛋白(IgG1、IgG2、IgG3、IgG4、IgA、SIgA、IgM)、淋巴细胞自噬蛋白Beclin1、自噬指标微管相关蛋白l轻链(LC3-Ⅱ)、磷脂酰肌醇三磷酸激酶(PI3K)、AKT、哺乳动物雷帕霉素靶蛋白(mTOR),观察上述指标的变化并对其相关因素进行分析。方法:选取39例强直性脊柱炎患者为AS组,另设20例健康体检者为健康对照组,采用ELISA方法检测2组血清免疫球蛋白(IgG1、IgG2、IgG3、IgG4、IgA、SIgA、IgM),运用Western blot方法检测2组淋巴细胞自噬蛋白Beclin1、LC3-Ⅱ、PI3K、AKT、mTOR的变化。结果:(1)2组免疫球蛋白比较,IgA、IgG1、IgG3较健康对照组显著升高(P<0.05或P<0.01)。(2)2组细胞自噬相关指标比较,PI3K、mTOR较健康对照组明显升高(P<0.05或P<0.01)。(3)相关性分析结果显示,AS患者PI3K与LC3-Ⅱ呈负相关。(4)相关性分析结果显示,AS患者IgG1、IgG2、IgG3、IgG4与mTOR呈正相关,SIgA与Beclin1呈正相关。结论:AS患者存在体液免疫功能的紊乱和细胞自噬指标的变化,且各项指标存在一定的相关性。
Objective:To observe the changes and analyze the related factors of serum immunoglobulin ( IgG1,IgG2,IgG3,IgG4,IgA,SIgA,IgM ) ,lymphocyte autophagy protein Beclinl,LC3-Ⅱ ,PI3K,AKT and mTOR in 39 patients with ankylosing spondylitis and 20 healthy subjects.Methods:Thirty nine cases of ankylosing spondylitis were selected as an AS group,and 20 healthy subjects were selected as a control group,using ELISA method to detect serum immunoglobulin ( IgG1,IgG2,IgG3,IgG4,IgA,SIgA,IgM ) of the two groups,and using Western blot to detect the changes of Beclin1,LC3- Ⅱ ,PI3K,AKT and mTOR.Results: (1) According to the comparison of immunoglobulin of the two groups,the levels Of IgA,IgG1 and IgG3 in the AS group were significantly higher than those in the control group ( P 〈 0.05 or P 〈 0.01 ) . (2) PI3K and mTOR of the AS group were obviously higher than those in the control group (P 〈 0.05 or P 〈 0.01 ) . (3) Correlation analysis showed that PI3K in the AS group was negatively correlated with LC3- Ⅱ. (4) Correlation analysis showed that IgG1,IgG2,IgG3 and IgG4 in the AS group were positively correlated with mTOR,and SIgA were positively correlated with Beclinl.Conclusion:There are changes of humoral immune function and autophagy index in patients with ankylosing spondylitis,and there is a certain correlation between the indexes.
出处
《风湿病与关节炎》
2017年第7期19-22,37,共5页
Rheumatism and Arthritis
基金
国家中医药重点学科中医痹病学建设项目(国中医药发[2009]30号)
安徽人口职业学院课题项目(ky2016005)
安徽省教育厅质量工程重点教学研究项目(2016jyxm0333)
关键词
脊柱炎
强直性
免疫球蛋白
自噬蛋白
自噬通路
spondylitis,ankylosing
immunoglobulin
autophagy protein
autophagy pathway