蝴蝶兰组织培养褐化抑制研究

Study on Inhibition of Tissue-Browning in Tissue Culture of Phalaenopsis amabilis

以蝴蝶兰大红袍花梗经诱导萌发生成的丛生芽为试验材料,对培养温度、活性炭、椰子汁和转接周期4个因素设置正交试验,比较不同处理对丛生芽增殖过程中抑制褐化的效果,筛选出抑制褐化的最优方法,为其工厂化生产提供理论参考。结果表明,活性炭能显著抑制褐化且对褐化的影响最大,各因素对褐化影响效应大小排序为活性炭>转接周期>温度>椰子汁;活性炭1.0 g/L、转接周期10 d、温度25℃、椰子汁浓度150 m L/L为抑制褐化的最优处理。

To provide theoretical reference for the factory production, used shoots induced from pedicels germination of Phalaenopsis amabilis Big Red Robe as experimental material, culture temperature, concentration of AC, coconut juice concentration and switching cycle were set up to carry out the orthogonal test,and effect of different treatment on the inhibition of tissue-browning effect of the proliferation of cluster buds was compared to screen the optimal method to inhibit tissue-browning.The results showed that AC could significantly inhibit browning and had the greatest influence on browning.The effects of various factors on the browning effect were sorted by the AC concentration 〉switching cycle 〉temperature 〉coconut juice concentration.The optimum treatment for browning inhibition was AC 1.0 g/L, switching cycle 10 d,temperature 25 ℃ and coconut juice concentration 150 mL/L.