含硫化合物对Methylosinus trichosporium OB3b生长和合成聚-β-羟基丁酸酯的影响

EFFECTS OF SULFUR COMPOUNDS ON THE GROWTH OF METHYLOSINUS TRICHOSPRIUM OB3b AND PHB PRODUCTION

为了将人类活动排放的含有甲烷的废气用于聚-β-羟基丁酸酯(PHB)合成过程,以M.trichosporium OB3b为菌种,考察硫化氢(H2S)和甲硫醚(DMS)对甲烷氧化菌生长和PHB合成的影响。实验结果表明,H2S浓度高达2 500μmol/mol时对甲烷氧化菌生长及甲烷单加氧酶(MMO)活性基本无影响,含H2S时的PHB含量和产率比无H2S时高,甲烷氧化菌经H2S培养后PHB合成能力增强。然而,DMS浓度为200μmol/mol时即可明显抑制甲烷氧化菌的生长及MMO活性。在DMS存在条件下,PHB含量和PHB产率都明显降低,经DMS培养后甲烷氧化菌的PHB含量进一步降低。

In order to produce poly-13-hydroxybutyrate （PHB） using waste methane emitted by anthropogenic activities, effects of hydrogen sulfide （H2S） and dimethyl sulfide （DMS） on the growth of Methylosinus trichosporium OB3b and PHB production were studied. The results showed that H2S with a concentration up to 2 500 p, mol/mol still had no effect on the growth of methanotroph and methane monooxygenase （MMO） activity. Compared with PHB production in the absence of H2S, both the PHB content and the PHB yield were elevated in the presence of H2S. PHB accumulation capacity of methanotroph was enhanced after cultivated with H2S. However, the growth and MMO activity of methanotroph was obviously depressed by DMS of 200 μmol/mol. Correspondingly, in the presence of DMS, the PHB content and the PHB yield were decreased significantly. The PHB content was further reduced with bacteria cultivated by DMS as inoculation.