Rab7基因沉默对巨噬细胞中R848激活的细胞因子及MAPK信号通路影响

Effects of Rab7 gene silencing on cytokine and MAPK signal pathway activated by R848 in macrophage

目的:本实验阐述了Rab7对Raw264.7巨噬细胞中R848激活TLR7(Toll like receptor-7)所产生的细胞因子的影响,并探讨Rab7对MAPK信号转导的影响。方法:使用Rab7刺激silence-Rab7-Raw264.7细胞,检测R848激活TLR7后产生的TNF-α、IL-6、IFN-α、IFN-β与IP-10,通过Western blot检测MAPK的磷酸化水平,分析Rab7对MAPK的信号转导的作用。结果:Rab7对TLR7激活后细胞因子的产生具有抑制作用,Rab7对MAPK信号通路具有抑制作用。结论:本实验结果进一步说明Rab7是TLR7信号转导通路的负向调控因子,并且参与到MAPK信号通路中。

Objective：To investigate the effect of Rab7 on cytokine induced by TLR7 （Toll like receptor-7） R848 activated in Raw264. 7, and discusses the influence of Rab7 on MAPK signal transduetion. Methods： TLR7 downstream cytokines such as TNF-ct, IL-6, IFN-a, IFN-I3 and IP-10 activated by R848 were detected through Q-PCR in Rab7 silenced mouse macrophages, and then analysis of phosphorylation of MAPK determined with Western blot showed the effect of Rab7 on signal transduction of MAPK. Results： Rab7 inhibit production of cytokine activated by TLR7, and also, Rab7 had an inhibitory effect on MAPK signal pathway. Conclusion： The experimental results further illustrate that the Rab7 isthe TLR7 signal transduction pathway negative regulatory factor, and to participate in MAPK signaling pathway.