苏铁总黄酮对Lewis肺癌模型小鼠VEGF、bFGF、HIF-1α、NF-κB表达的影响

Effects of total flavonoids from Cycas Revolute on expression of VEGF,bFGF,HIF-1α and NF-κB in model mice of Lewis lung cancer

目的:探讨苏铁总黄酮对Lewis肺癌模型小鼠血管内皮细胞生长因子(VEGF)、碱性成纤维细胞生长因子(b FGF)、低氧诱导因子-1α(HIF-1α)、核转录因子(NF-κB)表达的影响。方法:采用HE染色法观察肿瘤组织的病理形态变化,免疫组化法和Western blot法检测肿瘤组织中VEGF、b FGF、HIF-1α、NF-κB蛋白的表达,荧光定量PCR法检测肿瘤组织中VEGF、b FGF、HIF-1α、NF-κBmRNA的表达。结果:免疫组化法、Western blot法和荧光定量PCR法的检测结果显示,三者结果基本一致,与模型组比较,苏铁总黄酮各给药组VEGF、b FGF、HIF-1α、NF-κB基因表达量和对VEGF、b FGF、HIF-1α、NF-κB蛋白表达表达率均降低,差异具有显著性(P<0.05)。结论:苏铁总黄酮治疗肺癌的作用机制可能为通过抑制侵袭转移相关基因VEGF、b FGF、HIF-1α、NF-κB的表达,进一步抑制侵袭转移相关功能蛋白VEGF、b FGF、HIF-1α、NF-κB的表达,从而发挥抗肺癌侵袭转移作用。

Objective：To investigate the effects of total flavonoids from Cycas Revolute on expression of vascular endothelial growth factor （VEGF）, basic fibroblast growth factor （bFGF）, Hypoxia inducible factor-1α （HIF-1 α） and nuclear factor-κB（NF-κB） in model mice of Lewis lung cancer. Methods： The expressions of VEGF, bFGF, HIF-1α and NF-KB in tumor tissues were detected by immunohistochemistry and Western blot. The expression of VEGF, VEGF and NF-KB in tumor tissues were detected by fluorescent quantitative PCR. BFGF, HIF-1α and NF-κB mRNA were detected by immunohistochemistry. Results： The results of immunohis- tochemistry,Western blot and Real-time PCR showed that the results were basically the same, compared with model group, the expression of VEGF, bFGF, HIF-1α, NF-KB mRNA and the expression of VEGF, bFGF, HIF-1α and NF-KB were decreased, the difference was highly significant （P〈0. 05 ）. Conclusion： The mechanism of total flavonoids from Cycas Revolute in the treatment of lung cancer may be through inhibition of the expression of VEGF, bFGF, HIF-1 α, NF-κB in invasion and metastasis, and further inhibit the expression of VEGF, bFGF, HIF-1α and NF-KB in invasion and metastasis-related proteins, thus play a role of anti-lung cancer invasion and metastasis.