鼠双微体基因在肝癌组织中表达的意义

MDM2 expression in hepatocellular carcinoma tissue and its significance

目的:探讨肝癌中鼠双微体基因(MDM2)表达的临床病理意义。方法:免疫组织化学法检测MDM2在172例肝癌组织中的表达,分析MDM2表达与病人临床病理特征的关系,并对临床数据进行分析。log-rank行生存检验,Cox比例风险模型分析MDM2在肝癌组织中预后。siRNA下调Hep G2肝癌细胞MDM2蛋白,观察对HepG2细胞增殖、克隆形成影响。结果:肝癌病人中MDM2阳性率为53.2%,高MDM2表达与病人肝内淋巴结转移、肿瘤级别高、微血管浸润均有一定关系(P<0.05~P<0.01),与病人性别、年龄、乙型肝炎状态、肿瘤大小、包膜完整性、肿瘤是否多发、BCLC分期及肿瘤复发均无明显关系(P>0.05)。单因素分析显示高MDM2表达与病人较差的生存率有一定关系(P<0.05~P<0.01),而Cox比例风险模型分析提示高MDM2表达在肝癌生存预测中提示预后较差(P<0.01)。siRNA下调Hep G2肝癌细胞MDM2蛋白表达可抑制细胞增殖、克隆形成。结论:肝癌病人中MDM2高表达与肿瘤进展有关,MDM2可能与肝癌病人预后较差有关。

Objective：To investigate the clinicopathology significance of mouse double minute 2（MDM2） expression in hepatocellular cancer tissue.Methods：The expressions of MDM2 in 172 cases hepatocellular cancer tissues were detected using immunohistochemistry.The relationship between MDM2 expression and clinicopathology,and clinical data were analyzed.The survival of patients was tested using log-rank,and the prognosis of patients was analyzed using Cox proportional hazards model.The MDM2 protein expression in HepG2 hepatocarcinoma cells was silenced,the effect of which on the proliferation and colony formation of HepG2 cells were observed.Results：The positive rate of MDM2 in liver cancer patients was 53.2%.The high MDM2 expression was related to lymph node metastasis,tumor grade and microvascular invasion（P〈0.05 to P〈0.01）,and the high MDM2 expression was not significantly associated with the gender,age and hepatitis B status of patient,tumor size,membrane integrity,whether multiple tumor,BCLC staging and tumor recurrence（P〉0.05）.The univariate analysis showed that the high MDM2 expression was related to the poor survival rate（P〈0.05 to P〈0.01）,and the Cox proportional hazards model analysis showed the effect of high MDM2 expression in predicting the prognosis of patients with was poor hepatocellular cancer（P〈0.01）.The siRNA silencing the MDM2 expression in HepG2 hepatocellular cancer cells could inhibit the proliferation and colony formation of cells.Conclusions：The high MDM2 expression in patients with liver cancer is associated with tumor progression,MDM2 may be associated with the poor prognosis of patients.