大孔树脂法纯化红枣色素工艺研究

Purification of Red Pigment from Jujube Fruits Peel by Macroporous Resin

以吸附率和解析率为指标,通过静态吸附研究,从9种不同型号的大孔树脂中筛选出对红枣(Zizyphus jujube)皮红色素选择性良好的材料,并考察工艺参数对树脂吸附及解析枣皮红色素的影响,优化枣皮红色素的纯化条件。结果表明,X-5对红枣皮红色素具有良好的选择性,动态吸附及解析特性研究显示其最佳的纯化工艺参数为上样液浓度1.0 mg/mL,流速1.0 mL/min,pH 3.0,洗脱液采用60%乙醇,流速2.0 m L/min。采用此工艺条件,枣皮红色素的色价从12.8提高到32.4。综上所述,X-5型大孔树脂对枣皮红色素有良好的吸附与解析性能,适用于枣红色素的批量纯化。

To explore the optimal purification status, nine types of macroporous resins were used to investigate the properties of static absorption and desorption for the pigment purification, one type of macroporous resin was chosen to further investi-gate the dynamic properties of absorption and desorption for this pigment. The results demonstrated that X-5 was considered the most suitable resin for purification of red pigment from jujube fruit peels. The optimal absorption parameters for the pig-ment was at the concentration of 1.0 mg/mL with pH at 3.0 and the loading flow rate of 1.0 mL/min, and the optimal des-orption conditions were using 60% ethanol as solvent at the flow rate of 2.0 mL/min. The color value of the obtained red pigment by this process reached from 12.8 to 32.4. The results provided the optimal process parameters with macroporous resin X-5 for industrial purification of the red pigment from jujube fruits.