摘要
半边莲去叶带节茎段接种于MS基本培养基中,附加2,4—D、NAA、6—BA等不同浓度激素,获得了愈伤组织和完整植株。并建立了一个半边莲快速繁殖程序:(1)MS+6—BA4mg/L用于诱导丛芽的形成;(2)MS+NAA2mg/L用于诱导根的形成。完整植株移栽成活率可达95％。
The callus and complete plantlct were obtained by culturing the stems of Lobelia Chinesis Lour,with knots but without leaf in the MS Cultural medium with different level of 2,4-D,NAA and 6-BA.The procedure of rapid propagation of Lobelia chinesis Lour,was built.Firstly,MS+6-BA 4mg/L was employed for the formation of shoots.Secondly,MS+NAA 2mg/L was employed for the formation of roots,The survival rate of transplantation of the complete plantlet was 95%.
出处
《海南师范大学学报（自然科学版）》
CAS
1999年第1期110-113,共4页
Journal of Hainan Normal University（Natural Science）
基金
海南省自然科学基金(批准号:39409)
关键词
半边莲
愈伤组织
离休茎段
再生植株
快速繁殖
Lobelia Chinesis Lour.
Callus
in vitro Plantlet regeneration
rapid propagation
