乳香提取物对HL-60细胞株VEGF分泌及其Flt-1受体表达的影响

Effect of frankincense extract on VEGF and its receptor-1 expression in acute promyelocytic leukemia cell lines HL-60 cells

目的:探索不同浓度乳香提取物作用于HL-60细胞后,白血病细胞株HL-60细胞VEGF mRNA表达及VEGF蛋白分泌及受体Flt-1的变化。方法:MTT法检测乳香提取物对细胞增殖的抑制作用,选择适当的药物浓度及作用时间;RT-PCR法检测乳香提取物处理前后HL-60细胞中VEGF mRNA表达水平;Western Blot法检测乳香提取物处理前后HL-60细胞VEGF分泌和受体Flt-1蛋白表达水平。结果:乳香提取物在15.0 mg/L时在体外能下调HL-60细胞VEGF mRNA的表达和VEGF蛋白的分泌并呈时间与剂量依赖性(P<0.05)。结论:乳香提取物在体外可以抑制HL-60细胞分泌VEGF,同时抑制HL-60细胞内VEGF及其受体Flt-1 mRNA及蛋白表达下降,具有潜在抑制急性早幼粒细胞白血病血管新生的作用,是一种可供选择的抗白血病药物。

Objective To assess the effects of Frankincense extract on VEGF and Flt-1 expression in acute promyelocytic leukemia Cell Lines HL-60 cells.Methods HL-60 cells were treated with Frankincense extract,then cell proliferation were examined by the method of MTT.The level of VEGF-mRNA expression was distinguished by semi-quantitative RT-PCR technique.Western Blot was applied to examine the protein expression of VEGF and Flt-1.Results Within the concentration of(10.0~15.0) mg/L,Frankincense extract strongly inhibited proliferation of HL-60 cells.When the drug is 15.0 mg/L,α-boswellic acid can inhibit the mRNA expression of VEGF and the protein expression of VEGF and Flt-1.Conclusion Within a certain drug concentration,Frankincense extract can inhibit HL-60 cells proliferation.Its role of inhibiting angiogenesis maybe relate with down regulating the expression of VEGF and Flt-1.