GPI-PLD抗实验性大鼠动脉粥样硬化形成的初步研究

Antiatherogenic effect of GPI-PLD on experimental atherosclerosis in rats

目的:探讨糖基化磷脂酰肌醇特异性磷脂酶(GPI-PLD)对实验性动脉粥样硬化大鼠血脂水平及血管功能、形态的影响。方法:雄性大鼠随机分为三组,静脉输入生理盐水对照组、高表达GPI-PLD(+)细胞组和GPI-PLD(-)细胞组。高脂喂养与维生素D注射(60万IU/kg)建立大鼠动脉粥样硬化模型。实验第6周末,检测血脂水平(总胆固醇,甘油三酯,低密度脂蛋白、高密度脂蛋白)和血清GPI-PLD活性,观察主动脉病理形态学,检测主动脉对乙酰胆碱诱导的内皮依赖性舒张反应。结果:与生理盐水对照组、GPI-PLD(-)细胞组比较,输入高表达GPI-PLD(+)细胞组大鼠的血GPI-PLD活性增加,总胆固醇、甘油三酯、低密度脂蛋白量减少,而高密度脂蛋白增多(P<0.01);动脉内皮细胞较完整,血管增厚程度明显减轻,泡沫细胞数量明显减少;结论:GPI-PLD可抑制高脂喂养大鼠动脉粥样硬化形成。

Objective To investigate effects of the GPI-PLD activity on blood lipids and atherogenesis in the experimental atherosclerotic rats.Methods Ninty rats were randomly divided into three groups.The atherosclerotic model rats were injected with a single dose of vitamin D(600 000 IU/kg) and loaded with high fat diet.By the sixth weekend,the serum cholesterol,triglyceride,low densitylipoprote,high density lipoprote in cholesterol and GPI-PLD activity were determined.The aorta pathomorphism and vasodilator responses to acetycholine were observed.Results Compared with the saline control group,the contents of serum TC,TG and LDL was decreased.Meanwhile,The contents of serum HDL-c was increased significantly(P<0.01) in GPI-PLD(+) group.The aortic wall thickness was decreased and foam cells reduced significantly in GPI-PLD(+)group.Conclusion GPI-PLD can inhibit the atherosclerosis formation in rats.